Fluorescence detection of Al3+ ion in aqueous medium and live cell imaging by ESIPT probe (E)-N'-(5-bromo-2-hydroxybenzylidene)-4-hydroxybenzohydrazide

[Display omitted] •ESIPT probe (E)-N'-(5-bromo-2-hydroxybenzylidene)-4-hydroxybenzohydrazide (BHHB)•Al3+ ion sensor in the sub-nano molar scale by fluorescence enhancement in aqueous medium.•Imaging of nucleus of live cells by fluorescence confocal microscopy. The molecule (E)-N'-(5-bromo-...

Full description

Saved in:
Bibliographic Details
Published in:Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2023-09, Vol.297, p.122749, Article 122749
Main Authors: Bhakta, Viki, Shireen, Zofa, Dey, Sanjit, Guchhait, Nikhil
Format: Article
Language:English
Subjects:
Al3+ sensor
ESIPT
Fluorescence microscopy
HepG2 cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] •ESIPT probe (E)-N'-(5-bromo-2-hydroxybenzylidene)-4-hydroxybenzohydrazide (BHHB)•Al3+ ion sensor in the sub-nano molar scale by fluorescence enhancement in aqueous medium.•Imaging of nucleus of live cells by fluorescence confocal microscopy. The molecule (E)-N'-(5-bromo-2-hydroxybenzylidene)-4-hydroxybenzohydrazide (BHHB) has been synthesized and its photophysical properties have been investigated by using steady state absorption, emission and time resolved emission spectroscopy. The molecule shows excited state intramolecular proton transfer (ESIPT) process with characteristics large Stoke shifted emission. Fluorescence enhancement of BHHB only in presence of Al3+ ion is used as selective aluminium ion sensor in the sub-nano molar scale in aqueous solution. BHHB-Al3+ ion complex can penetrate through live Hepatocellular Carcinoma (HepG2) cell membranes and is capable for imaging of nucleus of live cells by fluorescence confocal microscopy.
ISSN:1386-1425
DOI:10.1016/j.saa.2023.122749