Spectroscopic characterization of eupalitin-3-O-β-D-galactopyranoside from Boerhavia procumbens: In vivo hepato-protective potential in rat model

[Display omitted] •Eupalitin-3-O-β-D-galactopyranoside compounds were isolated from Boerhavia procumbens.•Eupalitin-3-O-β-D-galactopyranoside rescued CCl4-induced liver via CD4 + Treg cell differentiation and LDH downregulation.•Molecular Docking shows LDH-blocking and FOXP3 expression pathways enha...

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Published in:Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2024-01, Vol.304, p.123369, Article 123369
Main Authors: Khalil, Abdul Wajid, Iqbal, Zafar, Adhikari, Achyut, Khan, Hamayun, Nishan, Umar, Iqbal, Anwar, Bangash, Javed Abbas, Tarar, Omer Mukhtar, Bilal, Ahmad, Khan, Shahid Ali, Hoessli, Daniel C., Assiri, Mohammed A., Wu, Zhiyuan, Afridi, Saifullah
Format: Article
Language:English
Subjects:
Hepatoprotective
In vivo rat model study
Secondary metabolites
Spectroscopic analysis
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Summary:[Display omitted] •Eupalitin-3-O-β-D-galactopyranoside compounds were isolated from Boerhavia procumbens.•Eupalitin-3-O-β-D-galactopyranoside rescued CCl4-induced liver via CD4 + Treg cell differentiation and LDH downregulation.•Molecular Docking shows LDH-blocking and FOXP3 expression pathways enhancement. The liver is one of the most important organs responsible for detoxifying biomolecules and xenobiotics. Herein, we report the isolation, characterization, and hepatoprotective effect of the Boerhavia procumbens-derived eupalitin-3-O-β-D-galactopyranoside (EGP) compound. The structure of the EGP compound was deduced by using NMR spectroscopic techniques and mass spectrometry. The EGP hepatoprotective activities were evaluated with HepG2 cell viability and LDH assays in vitro, and CCl4-induced toxicity was investigated in vivo in the rat model. Compared to the CCl4-treated group, cells exposed to the EGP compound at 200 µg/ml showed increased cell viability (60.52 ± 1.22 %) and decreased LDH levels (23.81 ± 1.89 U/ml). The serum levels of SGPT, SGOT, ALP, and total bilirubin in the CCl4-treated group were substantially higher than those in the control group (64 ± 1.89 U/ml, 86 ± 1.47 U/ml, 252.6 ± 2.96 U/ml, and 5.45 ± 0.32 mg/dl, respectively). When compared to animals treated with CCl4 alone, the EGP compound's in vivo hepatoprotective effect at 60 mg/kg with CCl4 significantly (p 
ISSN:1386-1425
DOI:10.1016/j.saa.2023.123369