Preparation of 3D nano silver trees/sea urchin-like gold and SERS detection of uric acid

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2024-01, Vol.305, p.123464, Article 123464
• Main Authors: Lin, Xin, Li, Li-Jun, Guo, He-Yuan-Xi, Li, Rui, Feng, Jun
• Format: Article
• Language: English
• Subjects: 3D nanosilver trees; Sea urchin-like nano gold; Surface Enhanced Raman Spectroscopy (SERS); Uric acid
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2023.123464

[Display omitted] •The substrate is a three-dimensional structure with a large specific surface area, which facilitates the construction of high-density hot spots.•Gold and silver nanoparticles both have a large number of nano-tips, making it easy to form hot spots.•The entire substrate preparation method is simple and easy to use, with direct detection of the substance to be measured. In this paper, 3D nano-silver trees/sea urchin-like gold Surface Enhanced Raman Spectroscopy (SERS) substrates were prepared by chemical reduction method, characterized by SEM, EDS, UV–Vis and XRD et.al, and the preparation process was optimized using Rhodamine B (RB) as the Raman signal molecule. The SERS characteristics of Uric Acid on this substrate were investigated and the results showed that the SERS substrate had a Raman enhancement factor of 1.9 × 107 for RB, and also had a significant Raman enhancement effect on Uric Acid. The SERS intensity (ISERS) at 1400 cm−1 showed a good linear relationship with the logarithm value of uric acid concentration (log C) between the range of 5 × 10−4 M to 1 × 10−7 M. The linear fitting equation was ISERS = 890 (log C) + 8066 (R2 = 0.983), and the LOD = 2.8 × 10−8 M. The spiked test was performed in human serum with recoveries ranging from 82.86% to 125.13% and RSD (n = 3)