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Quantification of ternary mixture of paracetamol, chlorzoxazone and ibuprofen present in tablet dosage form using ratio subtraction spectrophotometric approaches

[Display omitted] •The ternary mixture present in tablet formulation was quantitatively determined using RS, ERS and SRS methods.•The quantitative determination of each component was carried out using spectral manipulation without adopting separation process.•The developed method is precise, accurat...

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Published in:Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2024-04, Vol.310, p.123891, Article 123891
Main Authors: Mendonsa, Jaime, Gandhi, Santosh, Mahajan, Anand
Format: Article
Language:English
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Summary:[Display omitted] •The ternary mixture present in tablet formulation was quantitatively determined using RS, ERS and SRS methods.•The quantitative determination of each component was carried out using spectral manipulation without adopting separation process.•The developed method is precise, accurate and simple as compare to sophisticated chromatographic technique like HPLC.•The method can be adopted for routine analysis of marketed formulation in quality control laboratories. A sensitive and selective spectrophotometric approach comprising of successive ratio subtraction was developed for quantification and resolution of spectrum of mixture containing three components without prior separation. Three components, namely paracetamol, chlorzoxazone and ibuprofen were present in tablet dosage form. The linearity studies were carried out by recording zero order spectra and measuring absorbances at 285.0, 282.0 and 220.0 nm for paracetamol, chlorzoxazone and ibuprofen respectively. The drugs exhibited linear response in the concentration range of 6.0–18.0, 3.0–15.0 and 4.0–20.0 µg / mL for paracetamol, chlorzoxazone and ibuprofen respectively. The spectrum of paracetamol was most extended which was subsequently followed by moderately extended spectrum of chlorzoxazone and unextended spectrum of ibuprofen. The ratio spectra were manipulated successfully for quantification of paracetamol, chlorzoxazone, and ibuprofen. The developed method was validated as per ICH guidelines for the parameters of specificity, linearity, precision and accuracy. The percent recoveries for all the three drugs were in the range of 98.0–102.0 % with mean recovery of paracetamol, chlorzoxazone and ibuprofen were 99.72, 99.02 and 100.34 % respectively. Additionally the validity of the method is assured by analyzing marketed formulation.
ISSN:1386-1425
DOI:10.1016/j.saa.2024.123891