An eco-friendly one-pot spectrofluorimetric approach for the facile determination of overactive bladder drug, tolterodine: Application to dosage forms and biological fluids

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2024-04, Vol.311, p.123986, Article 123986
• Main Authors: Derayea, Sayed M., Badr El-Din, Khalid M., Ahmed, Ahmed S., Abdelshakour, Mohamed A., Oraby, Mohamed
• Format: Article
• Language: English
• Subjects: Fluorescence; Human plasma; Pharmaceutical analysis; Sodium dodecyl sulfate; Tolterodine
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2024.123986

Mechanism of the enhancement of tolterodine native fluorescence upon inclusion in the SDS micelle aggregate. [Display omitted] •A new, environmentally friendly spectrofluorimetric technique was developed to quantify tolterodine.•The method involves amplifying the drug’s native fluorescence by an impressive 720 %, using an aqueous SDS solution.•The developed approach proved highly effective in analyzing tablets as well as assessing tablets content uniformity.•Thanks to its high sensitivity, the proposed method was successfully applied to quantify the drug in spiked human plasma .•Compared with previous methods, the proposed approach offers high simplicity, sensitivity, eco-friendliness and versatility. Tolterodine tartrate (TTD) was the first antimuscarinic medication developed exclusively for the treatment of overactive bladder syndrome and was approved by the FDA in 1998. As a result of the drug's extensive utilization within the local community following its authorization, there is a pressing need to develop and validate a spectrofluorometric method that is economically efficient, easily reproducible, environmentally sustainable, and possesses high sensitivity. The developed approach relies on enhancing the fluorescence intensity of TTD to reach a level 720 % higher than its initial value, achieved through the application of an aqueous sodium dodecyl sulfate (SDS) solution. A strong correlation was observed with a correlation coefficient of 0.9998 between the concentration of TTD and the fluorescence intensity within the range of 25.0–500.0 ng mL−1. This approach could be employed to quantify TTD in its pure form and to examine pharmaceutical tablets for the purposes of verifying uniform content. Additionally, it was utilized for the evaluation of TTD concentrations in spiked human plasma.