A new FRET-based fluorescent probe: Colorimetric and ratiometric detection of hypochlorite and anti-counterfeiting applications

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2024-10, Vol.318, p.124477, Article 124477
• Main Authors: Ji, Peng, Liu, Yuntong, Li, Wanmeng, Guo, Ruixue, Xiong, Lingxiao, Song, Zhiguang, Wang, Bo, Feng, Guodong
• Format: Article
• Language: English
• Subjects: FRET; Hypochlorite; Ratiometric; Smartphone imaging
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2024.124477

[Display omitted] •A novel FRET ratiometric fluorescent probe (NDAC) for ClO− detection was designed and synthesized.•NDAC had good detection ability and excellent specificity for ClO−.•NDAC with smartphone-based fluorescence imaging analysis is applied for quantitative detection of ClO−.•NDAC has also been successfully used as an encryption dye in the field of information forgery. Hypochlorite (ClO−), as the main component of widely used disinfectants in daily life, comes into closer contact with the human body, which can lead to a number of diseases. The high-performance method is increasingly needed to detect ClO− in our daily life. In this report, we successfully synthesized a FRET ratiometric fluorescent probe (NDAC) containing benzoxadiazole moieties and coumarin moieties bound via ethylenediamine. As expected, NDAC has excellent selectivity and anti-interference ability toward ClO−, and the ratio of fluorescence intensity (I471 nm/I533 nm) has a very good linear relationship with the concentration of ClO−, with a wide linear range (2.5–1750 μM) and low detection limit (0.887 μM). Furthermore, we have successfully applied it for the quantitative detection of ClO− in water samples in daily life. At the same time, there is a very clear change in the fluorescence color after the reaction of the NDAC with ClO−. The blue/green value (B/G) of this color change also shows a very good linear relationship to ClO− (5.0–1000 μM). Therefore, the NDAC has also been successfully used for test strip detection and quantitative detection of ClO− in actual samples through smartphone-based fluorescence image analysis, and this method can provide faster, more convenient and more accessible detection. In addition, NDAC sensors also have potential applications in the field of information anti-counterfeiting.