A new SERS quantitative analysis strategy for ultratrace chloramphenicol with Fe3O4@MIP nanocatalytic probe

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Molecular and biomolecular spectroscopy, 2024-12, Vol.322, p.124732, Article 124732
• Main Authors: Huang, Haoyin, Wen, Guiqing, Liang, Aihui, Jiang, Zhiliang
• Format: Article
• Language: English
• Subjects: Chloramphenicol; Magnetic molecularly imprinted polymer; Nanocatalytic amplification; Separation and enrichment; Surface enhanced raman scattering quantitative analysis
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2024.124732

[Display omitted] •A novel magnetic nanosurface MIP nanoprobe (Fe3O4@MIP) was synthesized.•Fe3O4@MIP efficiently catalyzed the AuNP reaction of HAuCl4-fructose.•A sensitive SERS/RRS dimode method was established with Fe3O4@MIP probe.•Fe3O4@MIP can be separated by magnet, to improve the sensitivity. Three functional magnetic nanocatalytic probe, which integrates recognition, catalytic amplification, and separation enrichment, is a new approach to construct a simple, fast, highly selective, and sensitive analytical method. In this article, a new magnetic nanosurface molecularly imprinted polymer nanoprobe (Fe3O4@MIP) with trifunctionality was rapidly prepared using a microwave-assisted method with magnetic Fe3O4 nanoparticles as a substrate, chloramphenicol (CAP) as a template molecule, and methacrylic acid as a functional monomer. The characterized nanoprobe was found that could specifically recognize CAP, strongly catalyze the new indicator nanoreaction of fructose (DF)-HAuCl4. The gold nanoparticles (AuNPs) exhibit strong resonance Rayleigh scattering (RRS) and surface enhanced Raman scattering (SERS) effects. Upon addition of CAP, the SERS/RRS signals were linearly weakened. Accordingly, a new SERS/RRS analysis platform for highly sensitive and selective determination of CAP was constructed. The SERS linear range was 0.0125–0.1 nmol/L, with detection limit (DL) of 0.004 nmol/L CAP. Furthermore, it could be combined with magnet-enriched separation to further improve the sensitivity, with a DL of 0.04 pmol/L CAP. The SERS method has been used for the determination of CAP in real samples, with relative standard deviations of 2.37–9.89 % and the recovery of 95.24–107.1 %.