Monitoring transgene expression levels in different genotypes of field grown maize (Zea mays L.)

Transgenes in commercially available genetically modified plants are generally controlled by strong constitutive promoters to ensure a high level of expression at all stages of cultivation. Constitutive promoters however are influenced by a wide range of factors, and expression profiles of the trans...

Full description

Saved in:
Bibliographic Details
Published in:South African journal of botany 2013-01, Vol.84, p.6-10
Main Authors: Bakó, A., Gell, G., Zámbó, Á., Spitkó, T., Pók, I., Pintér, J., Balázs, E.
Format: Article
Language:English
Subjects:
backcrossing
corn
CP4 epsp
Cry3Bb1
gene expression
genetic background
Herbicide tolerance
herbicides
inbred lines
insect pests
leaves
Maize
monitoring
plant development
ribosomal RNA
statistical analysis
Transgene expression profile
transgenes
transgenic plants
Zea mays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Transgenes in commercially available genetically modified plants are generally controlled by strong constitutive promoters to ensure a high level of expression at all stages of cultivation. Constitutive promoters however are influenced by a wide range of factors, and expression profiles of the transgenes in multiple genetic backgrounds have not yet been extensively studied. In this study a powerful expression profiling methodology for transgenic maize (Zea mays L.) is demonstrated on a large scale, analysing thousands of data points from three genotypes of herbicide and insect pest tolerant transgenic maize. Martonvásár inbred lines were crossed with LH244 maize line containing the MON 88017 events, and leaf tissue from the sixth backcross generation was sampled at four relevant phenological phases. Relative expression levels were determined using 18S rRNA as a reference and detailed statistical analysis performed. Expression levels of both transgenes are varied throughout plant development, and the interaction between the genetic background and phenophase are significant (p
ISSN:0254-6299
1727-9321
DOI:10.1016/j.sajb.2012.09.005