Selecting salt-tolerant clones and evaluating genetic variability to obtain parents of new diploid and tetraploid germplasm in rhodesgrass (Chloris gayana K.)

• Published in: South African journal of botany 2013-01, Vol.84, p.88-93
• Main Authors: Ribotta, A.N., Griffa, S.M., Díaz, D., Carloni, E.J., López Colomba, E., Tommasino, E.A., Quiroga, M., Luna, C., Grunberg, K.
• Format: Article
• Language: English
• Subjects: AFLP; amplified fragment length polymorphism; Chloris gayana; Chloris gayana K; clones; diploidy; Genetic improvement; genetic variation; germplasm; hydroponics; parents; Salinity tolerance; salt stress; salt tolerance; sodium chloride; synthetic populations; tetraploidy
• ISSN: 0254-6299; 1727-9321
• DOI: 10.1016/j.sajb.2012.10.001

We evaluated survival percentage under salt stress in 46 diploid and tetraploid clones of rhodesgrass (Chloris gayana K.) with the aim of obtaining salt tolerant clones. Fifteen clones were selected at 600mM NaCl under hydroponic conditions. Survival percentage of the selected clones ranged between 50–100% and 50–75% for diploid and tetraploid clones, respectively. Genetic diversity among the 15 salt-tolerant clones was assessed using amplified fragment length polymorphism (AFLP). All tetraploid clones showed genetic diversity, whereas the diploid group included some genetically related clones. Clones tolerant at 600mM NaCl and showed genetic diversity are proposed as parents for new synthetic varieties of each rhodesgrass ploidy. ► Salt-tolerant rhodesgrass clones were obtained by survival percentage. ► Genetic variability in selected clones was assessed using the AFLP technique. ► AFLP discriminated tetraploid and diploid clones. ► Genetic variability in each ploidy level was observed. ► We selected clones as parents for new synthetic varieties.