Isolation, identification and characterization of pancreatic lipase inhibitors from Trigonella foenum-graecum seeds

• Published in: South African journal of botany 2019-03, Vol.121, p.418-421
• Main Authors: Fernando, W.I.T., Attanayake, A.M.K.C., Perera, H.K.I., Sivakanesan, R., Jayasinghe, L., Araya, H., Fujimoto, Y.
• Format: Article
• Language: English
• Subjects: Flavone C-glycoside; Isoschaftoside; Lipase inhibitor; Schaftoside; Trigonella foenum-graecum; Vicenin-1
• ISSN: 0254-6299; 1727-9321
• DOI: 10.1016/j.sajb.2018.10.023

Activity-guided fractionation of the methanol extract of Trigonella foenum-graecum seeds furnished three flavone C-glycosides having pancreatic lipase inhibitory activity. Structures of these compounds were established as vicenin-1 (1), isoschaftoside (2) and schaftoside (3) by detail analysis of 1H, 13C NMR and MS data. These compounds showed percentage inhibition 60.3% (1), 33.8% (2) and 95.5% (3) at the concentration of 250 μg/ml and IC50 values of the inhibition were 207 μg/ml (1), 330 μg/ml (2) and 130 μg/ml (3). This is the first report of the isolation of lipase inhibitors from T. foenum-graecum seeds. Temperature dependency of 1H NMR of 3 was studied in detail. •Lipase inhibitory activity of Trigonella foenum-graecum seeds were studied.•Three flavone C-glycosides were identified as lipase inhibitors.•Temperature dependency of 1H NMR of schaftoside was studied in detail.•Results proved the potential of T. foenum-graecum as a natural lipase inhibitor.