HPTLC quantification of diosgenin in Dioscorea deltoidea: Evaluation of extraction efficacy, organ selection, drying method and seasonal variation

• Published in: South African journal of botany 2021-05, Vol.138, p.386-393
• Main Authors: Nazir, Romaan, Kumar, Vijay, Dey, Abhijit, Pandey, Devendra Kumar
• Format: Article
• Language: English
• Subjects: Dioscorea deltoidea; Diosgenin; Green solvent; HPTLC; Microwave-assisted extraction; Seasonal variation
• ISSN: 0254-6299; 1727-9321
• DOI: 10.1016/j.sajb.2020.12.027

Dioscorea deltoidea (Dioscoreaceae) is an endangered species having tubers that possess anextensive range of therapeutic and economic value due to presence of bioactive compound diosgenin. Several industries have various necessities for compounds of specific classes and therefore, choosy extraction strategies for segregation of these classes ought to be produced.The current study aimed to screen potent part, identify best drying process, efficient extraction methods with solvent selection and seasonal variations on diosgenin content in D. deltoidea. The tuber contained the highest diosgenin content (1.15% DW) compared to leaf and stem. The highest yield of diosgenin was obtained using microwave-assisted extraction (MAE) with 50% ethanol (0.99% DW) and minimum incold maceration with water (0.46% DW). Shade drying was found to be the best drying method as compared to others in terms of diosgenin yield (1.43% DW) whereas sun drying produced low yield (1.12% DW). Estimation of yield and enriched diosgenin (1.19% DW) specified that winter (December) is the perfect time to harvest D. deltoidea. [Display omitted] •First report on drying methods, seasonal variations on diosgenin content in D. deltoidea.•First report on comparison of conventional and non-conventional methods for extracting diosgenin from D. deltoidea.•Microwave-assisted extraction (MAE) had higher extraction yield.