Variation in chemical constituents, in-vitro bioactivity and toxicity profile among different parts of Clerodendrum glandulosum Lindl. (C. colebrookianum Walp.)

In this study, chemical, biological and toxicity profile of hydromethanolic extracts of different parts (leaf, stem and root) of Clerodendrum glandulosum Lindl. were investigated simultaneously for the first time. Total phenolic and flavonoid contents were estimated spectrophotometrically. Untargete...

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Bibliographic Details
Published in:South African journal of botany 2021-08, Vol.140, p.50-61
Main Authors: Deb, Prashanta Kumar, Khound, Puspanjali, Bhattacharjee, Swarnali, Choudhury, Paramita, Sarma, Himanshu, Devi, Rajlakshmi, Sarkar, Biswatrish
Format: Article
Language:English
Subjects:
Antioxidant
Clerodendrum glandulosum
Enzyme inhibition
Secondary metabolites
Toxicity study
Verbascoside
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Summary:In this study, chemical, biological and toxicity profile of hydromethanolic extracts of different parts (leaf, stem and root) of Clerodendrum glandulosum Lindl. were investigated simultaneously for the first time. Total phenolic and flavonoid contents were estimated spectrophotometrically. Untargeted metabolomics was performed using GC–MS and HPLC-PDA-MS to identify the individual secondary metabolites. Antioxidant potentials (DPPH, ABTS, FRAP, phosphomolybdenum, superoxide ion scavenging assay) were estimated and enzyme inhibition assays (α-glucosidase, α-amylase, pancreatic lipase, xanthine oxidase, angiotensin-converting enzyme) were performed. Cytotoxicity of the extracts in HepG2 and L6 cell lines along with acute oral toxicity was carried out. Leaf extract exhibited the highest phenolic (282.02 ± 5.87 mg GAE/g) and flavonoid (276.74 ± 5.08 mg QCE/g) content. Significantly (p
ISSN:0254-6299
DOI:10.1016/j.sajb.2021.03.023