Investigation of therapeutic and immunomodulatory activity of Bacopa saponin from Bacopa monnieri

Nowadays, plants are potential bio-reactors for the manufacture of immunotherapeutic molecules. In this study, we investigated the effect of saponin (from Bacopa monnieri) against bacterial pathogens (Pseudomonas aeruginosa, Eschericia coli and Bacillus subtilis) and human peripheral blood mononucle...

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Bibliographic Details
Published in:South African journal of botany 2022-12, Vol.151, p.639-650
Main Authors: Pothiaraj, Govindan, Manoranjani, Murugan, Pitchaikani, Sasikumar, Seker, Gowtham Kumar, Saravanan, Konda Mani, Rajan, Mariappan, Shakila, Harshavardhan
Format: Article
Language:English
Subjects:
Bacopa monnieri
Bacopa saponin
Cytokine
Immunomodulator
PBMCs
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Summary:Nowadays, plants are potential bio-reactors for the manufacture of immunotherapeutic molecules. In this study, we investigated the effect of saponin (from Bacopa monnieri) against bacterial pathogens (Pseudomonas aeruginosa, Eschericia coli and Bacillus subtilis) and human peripheral blood mononuclear cells (PBMCs). The effect of Bacopa saponin was compared with commercial saponin for haemolytic activity, cytotoxicity and anti-inflammatory activity. In the haemolytic activity, more than 84% of haemolysis occurred in the high concentration (10 µg/mL) of Bacopa saponin. In the cytotoxic activity, 83% of cell viability was observed at 1 µg/mL concentration of Bacopa and commercial saponin and the 1 µg/mL concentration of bacopa and commercial saponin are induced into PBMCs and the expression of immune-related genes (IL2, IL6, IL8, CXCL12, CXCL17, IFN-ẟ, TNF-α, IL10, IL37, and IL19) were examined using real-time Polymerase Chain Reaction. This study observed that Bacopa saponin might be used as an immunomodulator and adjuvant.
ISSN:0254-6299
1727-9321
DOI:10.1016/j.sajb.2022.09.011