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Evaluation of the antioxidant and anti-ulcer activities of the ethanolic extract of Fumaria officinalis
Fumaria officinalis which belong to the Papaveraceae family is used to treat stomach-related ailments in Iraqi folk medicine. The aim of this study was to determine the anti-ulcer and antioxidant activities of a 70% ethanol extract of F. officinalis. Extracts of the aerial parts F. officinalis at do...
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Published in: | South African journal of botany 2022-12, Vol.151, p.816-825 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
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Online Access: | Get full text |
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Summary: | Fumaria officinalis which belong to the Papaveraceae family is used to treat stomach-related ailments in Iraqi folk medicine. The aim of this study was to determine the anti-ulcer and antioxidant activities of a 70% ethanol extract of F. officinalis. Extracts of the aerial parts F. officinalis at doses of 200 and 400 mg/kg body weight and the standard drug, esomeprazole at a dose of 20 mg/kg body weight were orally administered daily for 21 days after ulcer induction. The rats were sacrificed, and their stomachs were subjected to macroscopic and microscopic studies. The ethanolic extract showed a significant curative effect on ethanol-induced ulcer compared to the effects observed in the control and esomeprazole groups, especially at 400 mg/kg. Haematoxylin-eosin staining revealed remarkable improvement in gastric mucosal alteration, especially in rats treated with 400 mg/kg F. officinalis compared to the control. F. officinalis had high DPPH free radical scavenging activity (IC50: 189.2 ± 0.31 µg/mL) compared to ascorbic acid (215.5 ± 2.41 µg/mL). Further, HPLC analysis of the ethanolic extract revealed the presence of rutin, quercetin, ferulic, and p-coumaric acid in various quantities. In silico, rutin and quercetin were found to have the highest affinities of -9.4 and -7.6 kcal/mol, respectively with human H+/K+-ATPase α chain active sites, and the highest binding site energy values with Na+/K+-ATPase and pig gastric H+/K+- ATPase, respectively. Altogether, F. officinalis may be a potential candidate for the treatment of gastric ulcers.
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•Fumaria officinalis ethanolic extract treated ethanol-induced gastric ulcer.•Gastric ulcer healing mode of action of Fumaria officinalis involves inhibition H+/K+-ATPase, which is mediated mainly by rutin and quercetin.•In vitro antioxidant was assessed by DPPH, TPC and TFC assay.•HPLC analysis of ethanolic extract demonstrated the existence of flavonoids and phenolic acid in different quantities. |
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ISSN: | 0254-6299 1727-9321 |
DOI: | 10.1016/j.sajb.2022.11.008 |