Mass propagation of Satyrium nepalense D.Don.—A medicinal orchid via seed culture

An in vitro plant regeneration protocol was successfully established in Satyrium nepalense, a terrestrial orchid by culturing immature seeds from unripe fruits. Seeds were germinated on Murashige and Skoog, Knudson C and Knudson C modified Morel medium. The germination of the seeds and development o...

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Bibliographic Details
Published in:Scientia horticulturae 2009-01, Vol.119 (2), p.203-207
Main Authors: Mahendran, G., Bai, V. Narmatha
Format: Article
Language:English
Subjects:
1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea
acclimation
Agronomy. Soil science and plant productions
Asymbiotic seed germination
Biological and medical sciences
corms
culture media
cytokinins
explants
fruits (plant anatomy)
Fundamental and applied biological sciences. Psychology
growing media
in vitro regeneration
indole butyric acid
medicinal plants
micropropagation
Multiple shoots
Orchidaceae
plant development
plant growth substances
Protocorm
rooting
Satyrium nepalense
Seed culture
seed germination
seeds
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Summary:An in vitro plant regeneration protocol was successfully established in Satyrium nepalense, a terrestrial orchid by culturing immature seeds from unripe fruits. Seeds were germinated on Murashige and Skoog, Knudson C and Knudson C modified Morel medium. The germination of the seeds and development of protocorm was highest in MS medium (86.7%) followed by Knudson C modified Morel medium (74%) and Knudson C medium (61.2%). Among the cytokinins tried for multiple shoot induction from the protocorm, 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (TDZ) was found to be superior. Indole-3-butyric acid (IBA) was effective for inducing healthy roots. Well-developed plantlets were hardened in vermicompost (leaf litter + cow dung 1:1), sand and coconut husk (1:1:1).
ISSN:0304-4238
1879-1018
DOI:10.1016/j.scienta.2008.07.029