Selection of ABC genes for candidate genes of morphological changes in hydrangea floral organs induced by phytoplasma infection

• Published in: Scientia horticulturae 2009-11, Vol.122 (4), p.603-609
• Main Authors: Kitamura, Yoshikuni, Hosokawa, Munetaka, Uemachi, Tatsuya, Yazawa, Susumu
• Format: Article
• Language: English
• Subjects: ABC model; Agronomy. Soil science and plant productions; Bacterial plant pathogens; Biological and medical sciences; calyx; carpels; color; cultivars; Floral gene expression; Floral organs; flowers; Fundamental and applied biological sciences. Psychology; gene expression; Hydrangea; inflorescences; leaves; morphogenesis; Phytopathology. Animal pests. Plant and forest protection; Phytoplasma; phytoplasmal diseases; plant morphology; plant pathogenic bacteria; signs and symptoms (plants)
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2009.06.028

The mechanism underlying the green colouration of floral organs in phytoplasma-infected hydrangeas was studied. Elucidation of morphological changes of floral organs induced by phytoplasma infection and a comparison of the expression level of genes related to floral organ morphogenesis between phytoplasma-infected and non-infected hydrangea plants was conducted. Sixteen hydrangea ( Hydrangea spp.) cultivars were used to study structural changes in floral organs induced by Japanese Hydrangea Phyllody (JHP) phytoplasma infection. The homeotic conversion of sepals to leaves was observed in different number of florets between cultivars, and was observed in 75.8–100% of the decorative florets and 2.7–67.3% of the non-decorative florets of JHP phytoplasma-infected inflorescences. The homeotic conversion of carpels to leaves was observed in 1.2–17.9% of the decorative florets and 0.1–18.9% of the non-decorative florets of infected inflorescences. The expression levels of hydrangea orthologues of class A, B and C genes were suppressed in the infected inflorescences during the developmental stage in which no morphological differences in the internal structure of floral organs have been observed. The contribution of this suppression to homeotic conversion of sepals and carpels to leaves was suggested.