Identification of up-regulated genes in tea leaves under mild infestation of green leafhopper

► 21 unique genes up-regulated by leafhopper feeding were isolated from tea plants. ► Defense and defense-signaling genes were found. ► Cellular transport and oxidative stress-related genes were identified. ► Several novel genes involving plant resistance to herbivores were isolated. Transcriptional...

Full description

Saved in:
Bibliographic Details
Published in:Scientia horticulturae 2011-09, Vol.130 (2), p.476-481
Main Authors: Yang, Huimin, Xie, Suxia, Wang, Lei, Jing, Shengli, Zhu, Xiaopei, Li, Xianwen, Zeng, Wei, Yuan, Hongyu
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Camellia sinensis
Fundamental and applied biological sciences. Psychology
Gene expression
Green leafhopper
Insect–plant interaction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:► 21 unique genes up-regulated by leafhopper feeding were isolated from tea plants. ► Defense and defense-signaling genes were found. ► Cellular transport and oxidative stress-related genes were identified. ► Several novel genes involving plant resistance to herbivores were isolated. Transcriptional changes accompany induced resistance of plants against insect feeding, and monitoring transcriptional reorganization triggered in response to herbivory is an essential step for deciphering the molecular basis of the resistance. To isolate up-regulated genes in tea leaves under mild infestation of green leafhopper ( Empoasca vitis Göthe), a subtractive cDNA library was constructed using the suppression subtractive hybridization strategy. Subsequent differential screening, DNA sequencing analyses, and real-time PCR analyses identified 21 unique genes up-regulated by leafhopper feeding from the library. Among the genes likely to be directly involved in defense, those encoding pathogen-related protein and type 2 ribosome-inactivating protein were found in the subtractive library. In addition, genes involved in the CBL (calcineurin B-like)–CIPK (CBL-interacting protein kinases) pathway, cell wall fortification, secondary metabolite synthesis, water transport, and oxidative stress protection were identified in our experiment. Besides, a number of genes that have not been previously associated with defense responses against insects were isolated.
ISSN:0304-4238
1879-1018
DOI:10.1016/j.scienta.2011.07.023