Nitric oxide is involved in ethylene-induced adventitious root development in cucumber (Cucumis sativus L.) explants

• Published in: Scientia horticulturae 2017-01, Vol.215, p.65-71
• Main Authors: Xu, Xiao-Ting, Jin, Xin, Liao, Wei-Biao, Dawuda, Mohammed Mujitaba, Li, Xue-Ping, Wang, Meng, Niu, Li-Juan, Ren, Peng-Ju, Zhu, Yong-Chao
• Format: Article
• Language: English
• Subjects: Adventitious root development; Cucumber (Cucumis sativus L.); Ethylene (ETH); Nitric oxide (NO)
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2016.12.006

•ETH and NO do promote adventitious rooting.•NO is involved in ETH-induced adventitious root development.•ETH mediates NOS and NR activities and gene expression during adventitious rooting.•ETH up-regulates the expression of rooting-related target genes. Both ethylene (ETH) and nitric oxide (NO) are endogenous signaling molecules that regulate plant adventitious root development. The relationship between ETH and NO in that process remains poorly understood. In this report, our results show that the effects of ETH on adventitious rooting in cucumber (Cucumis Sativus L.) explants were dose-dependent, and 0.5μM ETH donor ethrel treatment was the most effective concentration to promote adventitious rooting. The positive effect of ETH could be reversed by NO scavenger 2-(4-carboxy-2-phenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO), nitric oxide synthase (NOS) inhibitor N-nitro-l-arginine methyl ester (L-NAME) or nitrate reductase (NR) inhibitor NaN3, indicating that the ETH-induced response, at least partially, was NO-dependent. Meanwhile, the exposure of cucumber explants to ETH up-regulated NOS and NR activity and their gene relative expression. Moreover, molecular evidence indicated that ETH improved the expression of genes involved in adventitious root development, including DNAJ-like gene (CsDNAJ-1) and calcium-dependent protein kinase genes (CsCDPK1 and CsCDPK5). However, the effects of ETH were inhibited by c-PTIO, L-NAME or NaN3. The results indicate that NO plays crucial role in the adventitious root development induced by ETH.