The roles of SlMYC2 in regulating ascorbate-glutathione cycle mediated by methyl jasmonate in postharvest tomato fruits under cold stress

• Published in: Scientia horticulturae 2021-10, Vol.288, p.110406, Article 110406
• Main Authors: Li, Zilong, Min, Dedong, Fu, Xiaodong, Zhao, Xiuming, Wang, Jihan, Zhang, Xinhua, Li, Fujun, Li, Xiaoan
• Format: Article
• Language: English
• Subjects: Ascorbate-glutathione cycle; Chilling tolerance; Methyl jasmonate; SlICE-SlCBF-SlCOR signaling pathway; SlMYC2; Tomato fruits
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2021.110406

lMeJA promoted ascorbate-glutathione (AsA-GSH) cycle in tomato under cold stress.lMeJA treatment activated the SlICE-SlCBF-SlCOR (ICC) signaling pathway.lSlMYC2 silence counteracted the effects of MeJA and aggravated the chilling injury.lSlMYC2 positively regulated MeJA-mediated AsA-GSH cycle and ICC signaling pathway. This study parsed the mechanism of SlMYC2 involving methyl jasmonate (MeJA)-mediated tomato fruit chilling tolerance. The data indicated that the application of MeJA significantly prevented chilling injury (CI) and inhibited the accumulation of hydrogen peroxide (H2O2) and superoxide anions (O2•−) in tomato fruit during cold storage. The accumulation of endogenous ascorbate (AsA) and glutathione (GSH) contents in fruit were enhanced by MeJA treatment resulting from increasing activities of enzymes related to AsA-GSH cycle. Meanwhile, the MeJA-treated fruit exhibited significantly higher expression levels of C-repeat-binding factor 1 (SlCBF1), cold regulated gene (SlCOR413) and inducer of SlCBF expression (SlICE1 and SlICEa), which belong to the SlICE-SlCBF-SlCOR (ICC) signaling pathway. However, the effects of MeJA were inhibited by the silence of SlMYC2. The expression levels of SlCBF1, SlCOR413, SlICE1 and SlICEa were reduced in SlMYC2-silenced fruits, and there was no significant difference in CI index and the indexes related to AsA-GSH cycle between (SlMYC2-silenced+MeJA)-treated fruit and control during most storage periods. In addition, correlation analysis indicated that the indexes involved in AsA-GSH cycle and ICC signaling pathway were closely related to the transcription of SlMYC2. Therefore, these results illustrated that SlMYC2 was involved in the regulation of ASA-GSH cycle and ICC signaling pathway mediated by MeJA during the cold tolerance of tomato fruit.