Genome- and transcriptome-wide systematic characterization of bZIP transcription factor family identifies promising members involved in abiotic stress response in sweetpotato

Basic region/leucine zipper (bZIP) transcription factors function as critical regulators in numerous abiotic stress responses. Massive bZIPs have been systematically identified in plants, but not in sweetpotato. In this study, 87 sweetpotato IbbZIPs unevenly distributed onto 15 chromosomes were iden...

Full description

Saved in:
Bibliographic Details
Published in:Scientia horticulturae 2022-09, Vol.303, p.111185, Article 111185
Main Authors: Liu, Siyuan, Zhang, Chengbin, Zhu, Qiwei, Guo, Fen, Chai, Ran, Wang, Minxin, Deng, Xiaoyao, Dong, Tingting, Meng, Xiaoqing, Zhu, Mingku
Format: Article
Language:English
Subjects:
Abiotic stress
bZIP transcription factor
Expression analysis
Sweetpotato
Systematic characterization
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Basic region/leucine zipper (bZIP) transcription factors function as critical regulators in numerous abiotic stress responses. Massive bZIPs have been systematically identified in plants, but not in sweetpotato. In this study, 87 sweetpotato IbbZIPs unevenly distributed onto 15 chromosomes were identified, and segmental duplications were shown to play dominant roles in the diversity of IbbZIPs. The collinearity between IbbZIPs and the related orthologs from nine plants depicted evolutionary insights into bZIP gene family. Additionally, IbbZIP proteins were clustered into 11 subfamilies while no members were found in the M subfamily, and the regularity of gene structures and conserved motifs was consistent with the subgroup classification. Evidence from transcriptome data and qRT-PCR analysis of 12 selected IbbZIPs revealed that they were significantly and varyingly induced under multiple abiotic stresses (salt, drought, heat and cold) and hormone treatments (ABA, ACC, JA and SA). Consistently, the promoters of IbbZIPs harbored multiple stress- and hormone-related cis-acting elements. Besides, IbbZIP17 and IbbZIP61 exhibited transactivation activity in yeasts, while IbbZIP-14/-69/-84 did not. These findings supply worthy foundations for further functional investigation of IbbZIPs, and multiple members may serve as promising regulators to enhance the abiotic stress tolerance of transgenic plants.
ISSN:0304-4238
1879-1018
DOI:10.1016/j.scienta.2022.111185