Toxic effects of ZnO nanoparticles towards marine algae Dunaliella tertiolecta

• Published in: The Science of the total environment 2013-02, Vol.445-446, p.371-376
• Main Authors: Manzo, Sonia, Miglietta, Maria Lucia, Rametta, Gabriella, Buono, Silvia, Di Francia, Girolamo
• Format: Article
• Language: English
• Subjects: Chlorophyta - drug effects; Chlorophyta - growth & development; Dunaliella tertiolecta; Environmental Exposure - analysis; Marine microalgae; Metal Nanoparticles - chemistry; Metal Nanoparticles - toxicity; Metal-oxide nanoparticles; Particle Size; Toxicity test; Zinc Oxide - chemistry; Zinc Oxide - toxicity; ZnO
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2012.12.051

Dose response curve and population growth rate alterations of marine Chlorophyte Dunaliella tertiolecta derived from the exposure to ZnO nanoparticles were evaluated. Bulk ZnO and ionic zinc were also investigated for comparison. At the same time, the aggregation state and particle size distribution were monitored. The evaluated 50% effect concentration (EC50 1.94 [0.78–2.31]mgZnL−1) indicates that nano ZnO is more toxic than its bulk counterpart (EC50 3.57 [2.77–4.80]mgZnL−1). Cross-referencing the toxicity parameters calculated for ZnCl2 (EC50 0.65 [0.36–0.70]mgZnL−1) and the dissolution properties of the ZnO, it can be gathered that the higher toxicity of nano ZnO is most likely related to the peculiar physicochemical properties of the nanostate with respect to the bulk material. Furthermore growth rate of D. tertiolecta was significantly affected by nano ZnO exposure. Our findings suggest that the primary particle size of the dispersed particles affect the overall toxicity. ► nano ZnO is more toxic for the marine alga D. tertiolecta than its bulk counterpart. ► nano ZnO affects the algal growth rate starting from lower tested concentrations. ► Toxic effects of nano ZnO cannot be explained only by the free zinc ion action. ► The nano ZnO primary size affects the overall toxicity.