Impacts of tannery effluent on development and morphological characters in a neotropical tadpole

• Published in: The Science of the total environment 2018-01, Vol.610-611, p.1595-1606
• Main Authors: Montalvão, Mateus Flores, da Silva Castro, André Luis, de Lima Rodrigues, Aline Sueli, de Oliveira Mendes, Bruna, Malafaia, Guilherme
• Format: Article
• Language: English
• Subjects: Amphibians; Animals; Anura - physiology; Aquatic xenobiotic; Development; Environmental Monitoring; Fluctuating asymmetry; Industrial Waste - analysis; Industrial Waste - statistics & numerical data; Larva - drug effects; Larva - growth & development; Metamorphosis, Biological - drug effects; Pollution; Tanning - statistics & numerical data; Waste Water - toxicity; Water Pollutants, Chemical - analysis; Water Pollutants, Chemical - toxicity
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2017.06.134

Previous studies have shown the contamination of rivers and streams caused by tannery effluents (TE). However, the effects of TE on amphibians are still unknown. Thus, we evaluated whether the TE, even at low concentrations (0.2%), could cause asymmetric development (inferred through the fluctuating asymmetry indices (FA)) and changes in morphological characteristics of Physalaemus cuvieri larvae. Accordingly, the tadpoles were distributed in groups non-exposed (n=160) and exposed (n=160) to this TE, for 15days. The TE used presented a complex chemical composition (especially high concentrations of Cr, Cd and Ni and a diverse array of toxic organic compounds). We used three individual FA indices: FA1:=|R-L|, ii) FA2=|R-L|÷[(R+L)÷2] and FA3=(R-L)2, where “R” are “L” are the right and left sides, respectively. The larvae exposed to the TE had higher FA indexes (FA1, FA2 and FA3) for nostril-snout distance, relative to the control group larvae. Regarding eye-snout distance, asymmetry values were higher for the FA1 and FA2 indices (with no difference for the FA3 index), which were similar to those observed for the eye-width character. For eye length, FA2 was higher in animals of the effluent group. Tadpoles exposed to TE had significantly lower body masses than those in the control group, when controlling for body length and larval development stage. Furthermore, tadpoles in the effluent group had smaller body size and lower interocular distance. The frequency of tadpoles that showed any alteration in tail morphology as well as tail attachment position (dorsal and ventral) was significantly higher in the effluent group than in animals not exposed to the TE. Finally, our data show that exposure to TE delayed larval development of tadpoles. Thus, our pioneering study confirms the hypothesis that exposure of P. cuvieri to TE, at low concentrations, impacts development and morphological characters. [Display omitted] •Tannery effluent (TE) decreases tadpole survival.•Tadpole exposed to TE presented changes in tail morphology.•Exposure to TE caused reduction of body mass and asymmetric development.•Water containing TE delayed larval development of tadpoles.