Dried blood spots for reliable biomonitoring of poly- and perfluoroalkyl substances (PFASs)

Dried blood spot (DBS) sampling has gained attention in several scientific areas because of the low sampling burden. The study aimed to develop a method for the determination of poly- and perfluoroalkyl substances (PFASs) in DBS using a standardized blood volume. The DBS method using a simple methan...

Full description

Saved in:
Bibliographic Details
Published in:The Science of the total environment 2019-03, Vol.655, p.1420-1426
Main Authors: Poothong, Somrutai, Papadopoulou, Eleni, Lundanes, Elsa, Padilla-Sánchez, Juan Antonio, Thomsen, Cathrine, Haug, Line Småstuen
Format: Article
Language:English
Subjects:
Adult
Chromatography, High Pressure Liquid - methods
DBS
Dried blood spot
Dried Blood Spot Testing - instrumentation
Dried Blood Spot Testing - methods
Environmental Monitoring - instrumentation
Environmental Monitoring - methods
Environmental Pollutants - analysis
Fluorocarbons - analysis
Humans
Middle Aged
Perfluoroalkyl substances
PFASs
Solid Phase Extraction - methods
Tandem Mass Spectrometry - methods
Whole blood
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Dried blood spot (DBS) sampling has gained attention in several scientific areas because of the low sampling burden. The study aimed to develop a method for the determination of poly- and perfluoroalkyl substances (PFASs) in DBS using a standardized blood volume. The DBS method using a simple methanol extraction followed by online solid phase extraction-ultra high performance liquid chromatography-tandem mass spectrometry quantification was validated. Only 30 μL of blood is required. Based on the measurements of DBS dispersed areas from known blood volumes (20–70 μL), the blood volume on a 3 mm diameter DBS subsample was calculated to be 3.3 μL (median, n = 708 measurements, 59 adults). Strong correlations of PFAS concentrations between finger prick DBSs and venous whole blood samples (n = 57) were found (rho 0.72–0.97, p 
ISSN:0048-9697
1879-1026
DOI:10.1016/j.scitotenv.2018.11.214