High-resolution mass spectrometry-based metabolomics reveal the disruption of jasmonic pathway in Arabidopsis thaliana upon copper oxide nanoparticle exposure

• Published in: The Science of the total environment 2019-11, Vol.693, p.133443, Article 133443
• Main Authors: Chavez Soria, Nita G., Bisson, Mary A., Atilla-Gokcumen, G. Ekin, Aga, Diana S.
• Format: Article
• Language: English
• Subjects: Arabidopsis - drug effects; Arabidopsis - metabolism; Arabidopsis thaliana; Chromatography, Liquid; Copper - adverse effects; CuO NPs; Cyclopentanes - metabolism; Jasmonates; LC-Q Exactive Orbitrap; LC-QToF; Mass Spectrometry; Metabolic Networks and Pathways; Metabolome - drug effects; Metabolomics; Metal Nanoparticles - adverse effects; Oxylipins - metabolism; Soil Pollutants - adverse effects
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2019.07.249

Mass-spectrometry based metabolomics has recently emerged as a valuable technique in understanding the ecotoxicity and mode of action of a wide range of xenobiotics in the environment, including engineered nanomaterials (ENMs). However, the applications of metabolomics in elucidating the biochemical pathways affected by xenobiotics have been mostly performed using targeted analysis. In this study, the effects of copper oxide nanoparticles (CuO NPs) on Arabidopsis thaliana, a model plant, was investigated using untargeted metabolite profiling based on two platforms of high-resolution mass spectrometry (MS): (1) liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QToF-MS) and (2) LC Q Exactive™ Hybrid Quadrupole-Orbitrap™-MS (LC-Orbitrap-MS). This approach was performed to identify specific features (mass-to-charge ratios, m/z's) that are significantly changed in a reproducible manner regardless of the MS platform used in metabolomics. In addition, the total copper concentrations taken up in plant tissues were quantified using inductively coupled plasma mass spectrometry (ICP-MS), which provided evidence of translocation of CuO NPs from roots to leaves and flowering shoots. Results from untargeted metabolomics showed that there were 65 plant metabolites that were altered commonly in both LC/MS platforms resulting from CuO NPs exposure of Arabidopsis thaliana. These metabolites belong to the jasmonic acid and glucosinolates pathways, suggesting the stress response induced by CuO NPs in Arabidopsis. This study demonstrated the effectiveness of high-resolution LC/MS in providing insight on the mechanism of nanotoxicity of CuO NPs in plants. [Display omitted] •Metabolomics was applied to investigate Arabidopsis thaliana response to CuO nanoparticles.•Translocation of copper to Arabidopsis thaliana shoots and leaves occurred.•65 metabolites changed based on two high-resolution mass spectrometry platforms.•Metabolites altered included isothiocyanates, scopoletin and jasmonates.