Microplastic (1 and 5 μm) exposure disturbs lifespan and intestine function in the nematode Caenorhabditis elegans

• Published in: The Science of the total environment 2020-02, Vol.705, p.135837, Article 135837
• Main Authors: Shang, Xu, Lu, Jiawei, Feng, Cheng, Ying, Yimeng, He, Yuanchen, Fang, Sheng, Lin, Ying, Dahlgren, Randy, Ju, Jingjuan
• Format: Article
• Language: English
• Subjects: Animals; C. elegans; Caenorhabditis elegans; Defecation; Ecosystem; Intestines; Lifespan; Longevity; Microplastics; Toxicity
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2019.135837

As an emerging environmental pollutant, microplastics (MPs) are increasingly viewed as a serious health concern to terrestrial and aquatic ecosystems. However, previous toxicological studies examining MPs on freshwater and terrestrial organisms provide contradictory results, possibly due to few investigations at environmentally relevant concentrations. Here, the nematode Caenorhabditis elegans (C. elegans), a model organisms with both aquatic and terrestrial free-living forms, was employed to investigate the effects of 1 and 5 μm MPs (107–1010 particles/m2) on the intake, lifespan, defecation rhythm, defecation-related neurons and transcriptional expression of related genes (skn-1, mkk-4, pmk-1, cpr-1 and itr-1). We demonstrated that the percentage of MP-contaminated nematodes increased with increasing exposure concentrations and duration. The lifespan of nematodes in the lower concentration exposure groups (2.4 × 107 and 2.4 × 108 particles/m2) decreased more prominently than that of higher concentration groups (2.4 × 109 and 2.4 × 1010 particles/m2) after a 72-h exposure period. Concomitantly, expression of the skn-1 gene, involved in detoxification and lifespan regulation, was significantly altered at lower MP concentrations. Physiologically, the defecation rhythm after a 72-h exposure period was most strongly affected by 1 μm MPs at 2.4 × 108 particles/m2. The significant up-regulation of related genes by 1 μm MPs appears responsible for the shortened defecation interval. Results of this study identified a potential toxicity threat to C. elegans from exposure to MPs at environmentally relevant concentrations and provide novel evidence for MP risks to freshwater and terrestrial organisms. Capsule. After exposure to 1 and 5 μm MPs (107–1010 particles/m2), the lifespan of C. elegans decreased more rapidly at lower concentrations. [Display omitted] •Toxicological effects of 1 and 5 μm microplastics (MPs) (107–1010 particles/m2) were studied on Caenorhabditis elegans.•Intake of 1 and 5 μm MPs increased as exposure concentrations and duration increased.•The lifespan of nematodes at lower concentration decreased faster than those at higher concentration after exposure to MPs.•Down-regulated expression of skn-1 at the lowest exposure concentration may be responsible for the shortened lifespan.