Methamphetamine exposure modulated oxidative status and altered the reproductive output in Daphnia magna

• Published in: The Science of the total environment 2020-06, Vol.721, p.137728, Article 137728
• Main Authors: De Felice, Beatrice, Mondellini, Simona, Salgueiro-González, Noelia, Castiglioni, Sara, Parolini, Marco
• Format: Article
• Language: English
• Subjects: Behavior; Illicit drugs; Methamphetamine; Oxidative stress
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2020.137728

Methamphetamine (METH) is a central nervous system stimulant drug whose use has increased in the last few years worldwide. After the ingestion of even a single dose, METH is excreted by the organism and enters the aquatic ecosystems, whereby concentrations up to hundreds of ng/L were measured in both sewage and surface waters. Although the environmental concentrations are currently quite low, the high biological activity of METH might cause adverse effects towards non-target organisms. However, to date the information on METH toxicity towards aquatic organisms is limited. Thus, the present study aimed at investigating biochemical and behavioral effects induced by METH exposure towards the Cladoceran Daphnia magna. A 21-days exposure to two environmental concentrations of METH (50 ng/L and 500 ng/L) was performed. At selected time points (7, 14 and 21 days) the amount of pro-oxidant molecules, the activity of antioxidant enzymes (SOD, CAT, GPx) and levels of lipid peroxidation (LPO) were measured as oxidative stress-related endpoints. Changes in swimming activity and reproductive output were assessed as behavioral endpoints. METH exposure affected the oxidative status of D. magna specimens at both tested concentrations, although no oxidative damage occurred. Although METH did not modulate the swimming activity of D. magna, a significant, positive effect on reproductive output, in terms of number of offspring was found. Our results showed that low concentrations of METH might represent a threat for D. magna, affecting the health status of this aquatic species at different level of biological organization. [Display omitted] •Methamphetamine toxicity was assessed by biochemical and behavioral endpoints.•Methamphetamine exposure imbalanced the oxidative status of D. magna.•Methamphetamine exposure did not induce any alteration in swimming behavior.•Increase in reproductive effort was observed from 50 ng/L upwards.