Driving factors influencing the rhizobacteriome community structure of plants adapted to multiple climatic stressors in edaphic savannas

• Published in: The Science of the total environment 2021-05, Vol.769, p.145214, Article 145214
• Main Authors: Thomas-Barry, Gem, St. Martin, Chaney C.G., Lynch, Michael D.J., Ramsubhag, Adesh, Rouse-Miller, Judy, Charles, Trevor C.
• Format: Article
• Language: English
• Subjects: Bacteriome; Biomass; Climatic changes; Core-microbiome; Ecosystem; Grassland; Plants; Rhizosphere; Root traits; Soil
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2021.145214

The natural variation of multiple abiotic stresses in hyper-seasonal edaphic savanna provides a unique opportunity to study the rhizobacteriome community structure of plants adapted to climate change-like conditions in the humid tropics. In this study, we evaluated changes in soil, plant and rhizobacteriome community structure parameters across seasons (wet and dry) in two edaphic savannas (SV-1 and SV-5) using four dominant plant species. We then examined relationships between rhizobacteriome community structure and soil properties, plant biomass, and conventional and novel root traits. We further hypothesized that plants adapted to the Aripo Savanna had a core rhizobacteriome, which was specific to plant species and related to root foraging traits. Our results showed that cation exchange capacity (CEC) and the concentration of micronutrients (Fe, Cu and B) were the only soil factors that differed across savanna and season, respectively. Plant biomass traits were generally higher in the dry season, with a higher allocation to root growth in SV-5. Root traits were more plastic in SV-5, and network length-distribution was the only root trait which showed a consistent pattern of lower values in the dry season for three of the dominant plant species. Rhizobacterial community compositions were dominated by Proteobacteria and Acidobacteria, as well as WPS-2, which is dominant in extreme environments. We identified a shared core rhizobacteriome across plant species and savannas. Cation exchange capacity was a major driver of rhizobacterial community assemblies across savannas. Savanna-specific drivers of rhizobacterial community assemblies included CEC and Fe for SV-1, and CEC, TDS, NH4+, NO3−, Mn, K, and network length-distribution for SV-5. Plant factors on the microbiome were minimal, and host selectivity was mediated by the seasonal changes. We conclude that edaphoclimatic factors (soil and season) are the key determinants influencing rhizobacteriome community structure in multiple stressed-environments, which are ecologically similar to the Aripo Savanna. [Display omitted] •Edaphoclimatic factors are the key determinants governing rhizobacteriome community structure.•The root trait network-length-distribution correlated with bacterial communities.•CEC was a common variable in explaining community structure.•Core-microbiome was not heavily influenced by plant and environmental factors.