Freely dissolved concentration profile and Hyalella azteca toxicity of cationic surfactant C12-benzalkonium in spiked-sediment toxicity test

• Published in: The Science of the total environment 2023-03, Vol.866, p.161226, Article 161226
• Main Authors: Yoshii, Sakura, Hiki, Kyoshiro, Watanabe, Haruna, Yamamoto, Hiroshi, Endo, Satoshi
• Format: Article
• Language: English
• Subjects: Amphipod; Ionic organic compounds; Passive sampling; Sediment chemical distribution; Sediment exposure indicator; Solid phase microextraction
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2022.161226

The freely dissolved concentrations (Cfree) have been considered a useful metric for exposure of aquatic organisms to organic contaminants. However, Cfree for cationic surfactants has rarely been measured, and its use in sediment toxicity tests has not been evaluated. In this study, Cfree of the cationic surfactant benzyldodecyldimethylammonium (C12-benzalkonium; C12-BAC) in water-only and spiked-sediment toxicity tests with the amphipod Hyalella azteca was analyzed using a passive sampling method. Polyacrylate-coated glass fibers were adopted as the passive sampler. Sorption isotherms of C12-BAC to the polyacrylate fibers were measured in chemical conditions comparable to those of the toxicity tests and used for Cfree calculation in both tests. Detailed concentration analysis in the sediment toxicity test demonstrated a high concentration gradient of C12-BAC between sediment and overlying water; Cfree in pore water was 17–78 times higher than Cfree in overlying water and was 7.2–13 times higher than Cfree at the sediment–water interface. The 50 % lethal concentration and bioconcentration factor of H. azteca obtained in the water-only test (23 μg/L and 140 ± 70 L/kg-wet, respectively) agreed with those calculated based on Cfree in pore water in the sediment test (49 μg/L and 140 ± 90 L/kg-wet, respectively), indicating that H. azteca is exposed mainly to the freely dissolved fraction in pore water. We concluded that Cfree in pore water is a useful exposure metric for H. azteca to cationic surfactants. [Display omitted] •Passive sampling enabled freely dissolved concentration (Cfree) analysis of C12-BAC.•pH and Ca2+ influenced the sorption isotherm of C12-BAC to a passive sampler.•There was a steep gradient of Cfree for C12-BAC in a sediment toxicity test system.•H. azteca was mainly exposed to Cfree of C12-BAC in pore water.•Cfree-based dose-response curves agreed in water-only and sediment exposure systems.