Ergothioneine attenuates the DNA damage of post-thawed Merino ram sperm

The objective of the current study was to evaluate the effects of antioxidant ergothioneine added to cryopreservation extender on the DNA integrity of Merino ram sperm. Semen samples from 5 mature Merino rams (1 and 2years of age) were used in the study. Semen samples, which were diluted with a Tris...

Full description

Saved in:
Bibliographic Details
Published in:Small ruminant research 2012-08, Vol.106 (2-3), p.165-167
Main Authors: Çoyan, Kenan, Bucak, Mustafa Numan, Başpınar, Nuri, Taşpınar, Mehmet, Aydos, Sena
Format: Article
Language:English
Subjects:
antioxidant activity
DNA
DNA damage
DNA integrity
Ergothioneine
Freezing–thawing
Merino
Ram sperm
rams
semen
semen extenders
spermatozoa
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The objective of the current study was to evaluate the effects of antioxidant ergothioneine added to cryopreservation extender on the DNA integrity of Merino ram sperm. Semen samples from 5 mature Merino rams (1 and 2years of age) were used in the study. Semen samples, which were diluted with a Tris-based extender containing ergothioneine at different concentrations and no antioxidant (control), were cooled to 5°C and frozen in 0.25ml French straws. Frozen straws were then thawed at 37°C for 20s in a water bath for evaluation of sperm DNA damage using the Comet test. The addition of ergothioneine at concentrations of 1, 2 and 4mM resulted in lower sperm with damaged DNA (5.4, 4.7 and 3.2%, respectively) than that of control (7.9%), thus reducing the DNA damage (P
ISSN:0921-4488
1879-0941
DOI:10.1016/j.smallrumres.2012.02.002