Evaluation of native 8kDa antigen based three immunoassays for diagnosis of cystic echinococcosis in sheep

• Published in: Small ruminant research 2014-02, Vol.116 (2-3), p.199-205
• Main Authors: Jeyathilakan, N., Abdul Basith, S., Lalitha John, Daniel Joy Chandran, N., Dhinakar Raj, G., Richard Churchill, R.
• Format: Article
• Language: English
• Subjects: 8 kDa antigen; CIEP; Cystic echinococcosis; EITB; LAT; Sheep
• ISSN: 0921-4488; 1879-0941
• DOI: 10.1016/j.smallrumres.2013.10.012

The dog tapeworm Echinococcus granulosus is the causative agent of cystic hydatid disease in domestic/wild herbivores animals and man. Accurate immunodiagnosis of the infection requires highly specific and sensitive antigens. The aim of this study was to develop and evaluate various immunoassays with principles of precipitation, agglutination and enzyme immunoassays for the identification of sheep infected with hydatid cyst which would allow the monitoring of animals from endemic areas and identifying infected animals prior to slaughter. The immunoassays were developed and validated using hydatid specific, non-cross reactive low molecular weight 8kDa hydatid cyst fluid protein. Sera used for the assay validations were obtained from 150 sheep infected naturally with hydatid cyst and 150 non-infected sheep. The highest diagnostic sensitivity was obtained in enzyme linked immune electro transfer blot (EITB) at 99.33 per cent followed by latex agglutination test (98.67 per cent) and counter immunoelectrophoresis (94.67 per cent). The study demonstrated that EITB was most sensitive immunological test for the detection of cystic echinococcosis in sheep. It should be useful for the conformation of hydatid cyst infected individual sheep. However, CIEP and LAT methods can be applied to practical use for screening studies.