Optimum dose and time of pregnant mare serum gonadotropin injections in Kacang goats to increase endogenous secretion of estrogen and progesterone without superovulation response

• Published in: Small ruminant research 2017-04, Vol.149, p.147-153
• Main Authors: Andriyanto, Amrozi, Rahminiwati, Min, Boediono, Arief, Manalu, Wasmen
• Format: Article
• Language: English
• Subjects: Corpus luteum; Estrogen; Follicle; Kacang goat; PMSG injection; Progesterone
• ISSN: 0921-4488; 1879-0941
• DOI: 10.1016/j.smallrumres.2017.02.005

•Doses of PMSG injection affect follicle growth and estradiol synthesis.•Doses of PMSG injection affect corpus luteum growth and progesterone synthesis.•Time of PMSG injection affects follicle growth and estradiol synthesis.•Time of PMSG injection affects corpus luteum growth and progesterone synthesis.•Injection of 7.5IU PMSG at luteolytic time gives the best result. The present study was conducted to determine what the optimum dose and time of pregnant mare serum gonadotropin (PMSG) injections in Kacang does to increase endogenous secretion of estrogen and progesterone without increasing the number of ovulating follicles above the normal range. Forty-eight Kacang does were assigned to a 3×4factorial randomized experimental design with 4 replications. The first factor was the dose of PMSG injection, and it consisted of three levels: 0, 7.5, and 15IU/kg body weight (BW). The second factor was the time of PMSG injection at the four stages of follicular development: the pre-luteolytic, luteolytic, pre-antral, and antral stages. The experimental goats were injected twice with prostaglandin to synchronize their estrous cycles. The parameters measured included the number and diameter of the dominant follicles and serum estrogen concentrations during the pre-ovulatory period and the number and diameter of corpora lutea and serum progesterone concentrations during the post-ovulatory period. The diameter of each dominant follicle and corpus luteum was also calculated. The results showed that an increased dose of PMSG injection significantly increased the numbers, total diameters, and diameter of each dominant follicle and corpus luteum, as well as serum estradiol and progesterone concentrations (P