Comparison of the bioactivity of whole and skimmed digested sheep milk with that of digested goat and cow milk in functional cell culture assays

• Published in: Small ruminant research 2017-04, Vol.149, p.202-208
• Main Authors: Mros, Sonya, Carne, Alan, Ha, Minh, Bekhit, Alaa El-Din, Young, Wayne, McConnell, Michelle
• Format: Article
• Language: English
• Subjects: Bioactivity; Cell culture assays; Cow; Goat; Milk; Sheep
• ISSN: 0921-4488; 1879-0941
• DOI: 10.1016/j.smallrumres.2017.02.018

•Sheep milk simulated gastric digests increase TNF- α production by THP-1 cells.•Sheep milk simulated gastric digests modulate mitogenic stimulation of splenocytes.•Cow milk simulated gastric digests increase IL-10 production by THP-1 cells. Milks from sheep, goat and cow were subjected to simulated gastric digestion prior to being analysed in cell culture assays to compare the bioactivity of the digests. All milks displayed a similar profile on 1D SDS-PAGE following digestion. Overall, the effects seen from the digested milks (whether whole or skimmed) in cell culture assays from the three animal species were similar with the exception of digested sheep milk which increased the amount of TNF-α produced by THP-1 cells in the presence of lipopolysaccharide (LPS) and digested cow milk which increased the amount of IL-10 produced by THP-1 cells in the presence of LPS. While sheep milk did not stimulate splenocyte proliferation directly, digested sheep milk had a greater effect on the response of splenocytes to mitogenic stimulation than cow and goat milk digests.