Metabolomic profile of young male goats seropositive to small ruminant lentivirus – A longitudinal study

• Published in: Small ruminant research 2019-05, Vol.174, p.135-140
• Main Authors: Milczarek, Maria, Czopowicz, Michał, Szaluś-Jordanow, Olga, Witkowski, Lucjan, Nalbert, Tomasz, Markowska-Daniel, Iwona, Bagnicka, Emilia, Puchała, Ryszard, Kosieradzka, Iwona, Kaba, Jarosław
• Format: Article
• Language: English
• Subjects: Caprine arthritis-encephalitis; Metabolomics; Seroconversion; Taurine
• ISSN: 0921-4488; 1879-0941
• DOI: 10.1016/j.smallrumres.2019.03.020

•Longitudinal study to compare metabolomic profile of SRLV-seropositive and negative male goats.•13 male goats followed up between the age of 6 months and 2 years.•134 different metabolites quantified for the first time in the serum of young male goats.•23 metabolites significantly differed between seropositive and seronegative goats.•96 metabolites tended to change significantly in time during first 2 years of goats’ life. We carried out a longitudinal study to determine the change of concentration of a large set of metabolites over the first 2 years of goats’ life and identify metabolites whose concentration follows different patterns in small ruminant lentivirus(SRLV)-infected and uninfected goats. Thirteen clinically healthy male goats, born to SRLV-infected does and castrated at the age of 4 months, were followed up for 2 years. They were serologically tested weekly and on this basis assigned to seropositive (n = 7) and seronegative (n = 6) group. At the age of 6, 12, 18 and 24 months their serum was subjected to metabolomic analysis using a commercial AbsoluteIDQ® p180 Kit assay designed to measure concentrations of 188 metabolites. Finally, 134 metabolites had concentrations above the limit of detection and were included in the analysis. Twenty three metabolites differed between seropositive and seronegative goats at a false discovery rate of 25% and with fold change of at least 1.5: 21 metabolites at the age of 6 months and 2 metabolites at the age of 24 months. Concentration of 96 metabolites tended to change significantly in time: concentration of 57 metabolites increased, 12 decreased, and 27 fluctuated. Concluding, SRLV infection affects metabolomic profile of young male goats.