Malate quinone oxidoreductase biosensors based on tetrathiafulvalene and gold nanoparticles modified screen-printed carbon electrodes for malic acid determination in wine

Screen-printed carbon electrodes have been modified with gold nanoparticles, tetrathiafulvalene and malate quinone oxidoreductase enzyme for the sensitive and selective detection of malic acid. Amperometric experimental conditions were optimized taking into account the importance of quantifying mali...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2014-10, Vol.202, p.971-975
Main Authors: Molinero-Abad, Begoña, Alonso-Lomillo, M. Asunción, Domínguez-Renedo, Olga, Arcos-Martínez, M. Julia
Format: Article
Language:English
Subjects:
Biosensor
Malate quinone oxidoreductase
Malic acid
Screen-printed carbon electrodes
Tetrathiafulvalene
Wine
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Summary:Screen-printed carbon electrodes have been modified with gold nanoparticles, tetrathiafulvalene and malate quinone oxidoreductase enzyme for the sensitive and selective detection of malic acid. Amperometric experimental conditions were optimized taking into account the importance of quantifying malic acid in wine samples and the inherent complexity of these samples, particularly red wine. The biosensor responds to malic acid giving an anodic current (at +100mV and pH 6.5) in a wide concentration range, with a capability of detection of 2.0μM (α=β=0.05) at room temperature. The method has been applied to the determination of malic acid in white, rose and red wine samples, with averages recoveries of 99.6%.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2014.06.057