A photothermal biosensor for detection of C-reactive protein in human saliva

•We develop a novel PTB system to measure the concentration of salivary CRP.•We are able to ascertain that the R2 value of the PTB is as high as that of the ELISA kit.•It is more convenient to perform measurements with the PTB than with the ELISA. In this study, our objective was to develop a photot...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2017-07, Vol.246, p.471-476
Main Authors: Lee, Seung Hyeon, Choi, Seoyeon, Kwon, Kirok, Bae, Nam-Ho, Kwak, Bong Seop, Cho, Won Chang, Lee, Seok Jae, Jung, Hyo-Il
Format: Article
Language:English
Subjects:
C-reactive protein
Gold nanoparticles
Human saliva
Photothermal biosensor
Triton X-100
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Summary:•We develop a novel PTB system to measure the concentration of salivary CRP.•We are able to ascertain that the R2 value of the PTB is as high as that of the ELISA kit.•It is more convenient to perform measurements with the PTB than with the ELISA. In this study, our objective was to develop a photothermal biosensor (PTB) for measurement of C-reactive protein (CRP) concentrations in human saliva. The resulting PTB that we developed is rapid, simple, and minimally invasive, and can be used to measure the concentration of CRP in human saliva (7-μL sample volume). The CRP (0.1–100ng/mL) is captured by the sandwich immunoassay on the sensor, and gold nanoparticles (GNPs) conjugated with antibodies cause a photothermal effect when irradiated with a laser (90s, wavelength=532nm). The temperature changes caused by this effect correlate with the concentrations of CRP. Therefore, the sensor shows a higher correlation with the concentrations of CRP than commercial detection kits. Its detection limit is 0.1ng/mL CRP.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2017.01.188