Construction of inorganic-organic cascade enzymes biosensor based on gradient polysulfone hollow fiber membrane for glucose detection

• Published in: Sensors and actuators. B, Chemical Chemical, 2023-06, Vol.385, p.133630, Article 133630
• Main Authors: Zhang, Chengxiong, Wei, Chenjie, Chen, Dajing, Xu, Zhikang, Huang, Xiaojun
• Format: Article
• Language: English
• Subjects: Cascade reaction; Glucose detection; Point-of-care testing; Polysulfone hollow fiber membrane; Reusable colorimetric biosensor
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/j.snb.2023.133630

The non-invasive diabetes sensing remains a widespread concern. Prussian blue nanoparticles (PBN) decorated with high superiority of enzymatic specificity, high catalytic activity, high stability, and low cost, can be operated in the field of enzyme-based sensing. Meanwhile, the glucose oxidase micro-particles (GOm) showed higher stability and reusability than the free glucose oxidase (GOx). Herein, a convenient PBN and GOm decorated hollow fiber membrane (PBN-GOm@HFM) colorimetric biosensor was developed through loading inorganic-organic cascade enzymes on the inner surface of polysulfone (PSF) HFM. Instead of complex testing methods and long incubation times, PB-GOm@HFM could be used for one-step glucose detection, and the linear range was found from 0.1 to 10 mM with a detection limit of 0.029 mM. The biosensor also showed excellent reusability and chemical stability (the initial activity decreased less than 10 % even after 20 cycles of detection glucose and the long-term activity stability remained over 80 % after storing for 4 weeks at 4 °C). Furthermore, the biosensor exhibited excellent semiquantitative non-invasive sensing testing for glucose in urine by the naked eye based on the siphon effect of HFM. •PBN and GOm were uniformly and stably loaded on the inner surface of PSF HFM.•The inorganic-organic cascade enzymes for glucose detection were achieved.•The biosensor showed excellent reusability and stability.•The biosensor could be extended to non-invasive color detection of body fluids by the naked eye.