Rapid microfluidic-thromboelastography (μ-TEG) for evaluating whole blood coagulation and fibrinolysis at elevated shear rates

The assessment of hemostasis and fibrinolysis is essential in emergency settings to make timely medical decisions. While conventional thromboelastography (TEG) analyzers provide comprehensive information on blood clotting, their long test times are often unsuitable for emergency patients. In this st...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2023-09, Vol.390, p.133873, Article 133873
Main Authors: Wang, Jikang, Choi, Cheol Ung, Shin, Sehyun
Format: Article
Language:English
Subjects:
Coagulation
Fibrinolysis
Microfluidic
Shear rates
Thromboelastography
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Summary:The assessment of hemostasis and fibrinolysis is essential in emergency settings to make timely medical decisions. While conventional thromboelastography (TEG) analyzers provide comprehensive information on blood clotting, their long test times are often unsuitable for emergency patients. In this study, we propose an innovative method of microfluidic thromboelastography (μ-TEG) that rapidly measures the viscoelastic properties of blood coagulation in oscillating flow through circular tubes. Our μ-TEG system moves a blood sample back and forth through a circular tube subjected to sinusoidal pressure, generating shear rates ranging from 1.2 to 20 s−1, higher than those of conventional TEGs. The movements are monitored by a pressure sensor and converted to a conventional TEG. Key parameters such as MA and Ly30 can be acquired within 10–12 min for normal samples. Our study showed that the increased shear rate in the μ-TEG system alters the coagulation mechanism, resulting in increased coagulation strength and rapid fibrinolysis. Despite the short test time, the μ-TEG showed good agreement with the TEG5000. The rapid measurement of μ-TEG makes it a useful tool for analyzing hemostasis and fibrinolysis in emergency and operating rooms. [Display omitted] •The μ-TEG system can measure the viscoelastic properties of blood clots within 10 min.•Oscillatory flow monitoring of blood coagulation successfully translated into TEG-equivalent results.•Elevated shear rate accelerates the coagulation and fibrinolysis processes, while also enhancing clot strength and fibrinolytic capacity.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2023.133873