Dual-signal immunosensor based on aggregation-induced electrochemiluminescence resonance energy transfer of La-MOF and low cathodic excitation potential of g-C3N4@AuNPs for the quantitation of human serum amyloid A

• Published in: Sensors and actuators. B, Chemical Chemical, 2025-02, Vol.424, p.136925, Article 136925
• Main Authors: Hu, Shenglan, Xu, Lixin, Huang, Minlan, Wu, Yusheng, Qin, Xuemin, Deng, Biyang
• Format: Article
• Language: English
• Subjects: Aggregation-induced electrochemiluminescence; Dual-signal immunosensor; Lanthanum-based metal-organic framework; Resonance energy transfer; Serum amyloid A
• ISSN: 0925-4005
• DOI: 10.1016/j.snb.2024.136925

We report on the development of a dual-signal electrochemiluminescence (ECL) immunosensor for the quantitation of human serum amyloid A (SAA). The immunosensor comprises g-C3N4@AuNPs as the cathode and a lanthanum-based metal organic framework (La-MOF) as the anode, the latter of which utilizes aggregation-induced electrochemiluminescence (AIECL) to overcome the otherwise poor ECL intensity of H4TBAPy ligands at the electrode surface. Furthermore, the rigid MOF structure significantly improved the fluorescence quantum yield, as well as the fluorescence and ECL emission, of the La-MOF compared to H4TBAPy. The g-C3N4@AuNPs exhibits an ultrathin nanosheet structure with a low cathodic excitation potential of −0.72 V. When the dual-signal ECL immunosensor was employed to detect SAA, the difference between the anodic and cathodic ECL signals exhibited a strong linear relationship with the logarithm of the SAA concentration in the range of 100 fg/mL–200 ng/mL, with a detection limit of 24.5 fg/mL. [Display omitted] •La-MOF exhibited excellent aggregation-induced electrochemiluminescence in anode.•ECL-RET between g-C3N4@AuNPs and La-MOF was developed•The dual-signal immunosensor showed excellent stability for SAA detection.