Trapping of phosphate solubilizing bacteria on hyphae of the arbuscular mycorrhizal fungus Rhizophagus irregularis DAOM 197198

• Published in: Soil biology & biochemistry 2015-11, Vol.90, p.1-9
• Main Authors: Taktek, Salma, Trépanier, Martin, Servin, Paola Magallon, St-Arnaud, Marc, Piché, Yves, Fortin, J.-André, Antoun, Hani
• Format: Article
• Language: English
• Subjects: AMF extraradical hyphae; Burkholderia; Glomus irregulare; Hyphosphere; Phosphate rock solubilization; Rhizobacteria
• ISSN: 0038-0717; 1879-3428
• DOI: 10.1016/j.soilbio.2015.07.016

A simple method is described for trapping phosphate solubilizing bacteria (PSB) strongly attached to the hyphae of the arbuscular mycorrhizal fungus (AMF) Rhizophagus irregularis (Ri). Bacteria were isolated from the hyphosphere of mycorrhizal leek plants growing on Turface previously inoculated with soil suspensions, obtained from the mycorrhizosphere of mycorrhizal plants growing in agricultural settings or maple forests in Quebec, Canada. Among the best PSB strongly attached to the hyphae of Ri, 26 isolates belonged to Burkholderia spp. and one was identified as Rhizobium miluonense. Four hyphobacteria exhibiting high potential of inorganic and organic P mobilization were further compared with four equivalent mycorrhizobacteria directly isolated from mycorrhizospheric soils sampled. In general, hyphobacteria were superior in mobilizing P from hydroxyapatite and from a low reactivity igneous phosphate rock from Quebec. Release of gluconic acid or the product of its oxidation 2-ketogluconic acid, are the main mechanisms involved in P solubilization. In a two compartments Petri plate system, Ri extraradical hyphal exudates, supported PSB growth and activity. In the absence of PSB Ri showed a negligible P solubilization activity. In the presence of PSB a substantial increase in P mobilization was observed, and the superiority of hyphobacterial activity was also observed under this system. Our results suggest that in developing a bioinoculant based on selected PSB, their interaction with AMF hyphae should not be overlooked. •Isolation of phosphate solubilizing bacteria from Rhizophagus irregularis hyphae is described.•Majority of bacteria strongly trapped on hyphae belong to Burkholderia spp.•Hyphobacteria are superior phosphate solubilizers than mycorrhizobacteria.•R. irregularis hyphal exudates can support growth and activity of bacteria.