Anabolic/anticatabolic and adaptogenic effects of 24-epibrassinolide on Lupinus angustifolius: Causes and consequences

• Published in: Steroids 2020-02, Vol.154, p.108545, Article 108545
• Main Authors: Kandelinskaya, Olga L., Grischenko, Helena R., Кhripach, Vladimir A., Zhabinskii, Vladimir N., Kartizhova, Lylia E., Shashko, Yuriy K., Kosmachevskaya, Olga V., Nasybullina, Elvira I., Topunov, Alexey F.
• Format: Article
• Language: English
• Subjects: Anabolic/anticatabolic action; Animals; Antifungal Agents - chemistry; Antifungal Agents - metabolism; Antifungal Agents - pharmacology; Brassinosteroids; Brassinosteroids - chemistry; Brassinosteroids - metabolism; Brassinosteroids - pharmacology; Coleoptera - drug effects; Colletotrichum - drug effects; Environmental sustainability; Insecticides - chemistry; Insecticides - metabolism; Insecticides - pharmacology; Lupine; Lupinus - chemistry; Lupinus - metabolism; Microbial Sensitivity Tests; N2-fixing system; Plant Growth Regulators - chemistry; Plant Growth Regulators - metabolism; Plant Growth Regulators - pharmacology; Protein biosynthesis; Seeds - chemistry; Seeds - metabolism; Spodoptera - drug effects; Steroids, Heterocyclic - chemistry; Steroids, Heterocyclic - metabolism; Steroids, Heterocyclic - pharmacology
• ISSN: 0039-128X; 1878-5867
• DOI: 10.1016/j.steroids.2019.108545

•Epibrassinolide possesses an anabolic effect on the nitrogen-fixing system of lupine.•Epibrassinolide has an anti-catabolic effect in the lupine ripening seeds.•Epibrassinolide increases resistance of lupine plants to phytopathogenes. Lupinus angustifolius L. is a legume culture known as a source of valuable feed protein and the N2-fixator for improving soil fertility. However, its low ecological resistance does not allow for a stable yield of the crop. Earlier, we have shown that steroid phytohormone 24-epibrassinolide (EBR) increases the tolerance of lupine to chlorine ions by activating the protective proteins in ripening seeds (such as proteinase inhibitors that prevent protein breakdown) and lectins. Here we investigated the effect of EBR on the functional status of the N2-fixing system in root nodules, protein synthesis in ripening seeds and the resistance of lupine plants to various pathogens. It was found that EBR enhanced the nodulation process, N2-fixing activity of nitrogenase and the accumulation of poly-β-hydroxybutirate in the bacteroides, increased the leghemoglobin content in the nodules as well as the metabolic activity of bacteroides. According to data on the inclusion of 14C-leucine in maturing seed proteins, EBR increased the accumulation of protein in them against the background of a short-term decrease in protein synthesis and its subsequent regeneration to the control level. Gradual inhibition of protein synthesis, characteristic of other legumes, was observed in control variants of lupine. EBR increased lupine resistance to phytopathogenic fungi of Colletotrichum genus and insects of Noctuidae and Scarabaeidae families. We concluded that a more complete implementation of the potential productivity and sustainability of lupine under the action of EBR was achieved due to the anabolic/anti-catabolic effect on the N2 fixation system in root nodules, as well as on protein synthesis in ripening seeds.