Enantiomeric separation of 1,4-dihydropyridines by liquid-phase microextraction with supercritical fluid chromatography

• Published in: The Journal of supercritical fluids 2016-01, Vol.107, p.129-136
• Main Authors: Zhang, Lisha, Song, Zhaorui, Dong, Yaqing, Wang, Yufen, Li, Xiaogang, Long, Hao, Xu, Kun, Deng, Chuan, Meng, Meng, Yin, Yongmei, Xi, Rimo
• Format: Article
• Language: English
• Subjects: 1,4-Dihydropyridines; Chiral separation; Enantiomers; Hollow fibre-based liquid-phase microextraction; Supercritical fluid chromatography
• ISSN: 0896-8446; 1872-8162
• DOI: 10.1016/j.supflu.2015.08.021

•A 2p-HF-LPME based chiral SFC for 1,4-DHP was developed.•Several 1,4-DHP enantiomers could be well separated.•The method is rapid, practical, effective and environmentally friendly. Chiral separation of seven commonly used 1,4-dihydropyridines (1,4-DHP) was achieved by supercritical fluid chromatography (SFC) on a immobilised polysaccharide chiral selectors coated with cellulose-tris(3,5-dichlorophenylcarbamate) (Chiralpak IC). In this method, isopropanol was used as a modifier and a maximum resolution of 13.38 was resulted. Nimodipine content of actual samples was determined through two-phase hollow fibre-based liquid-phase microextraction (2p-HF-LPME). Under optimal conditions, the limits of detection of the two nimodipine enantiomers were 0.3 and 0.5μgcm−3. Recoveries of 80.0–99.8% were achieved. The developed SFC technique coupled with 2p-HF-LPME is a rapid, effective and environment-friendly method for separating and quantifying 1,4-DHP enantiomers.