Development of functionalized alginate dressing with mango polyphenols by supercritical technique to be employed as an antidiabetic transdermal system

• Published in: The Journal of supercritical fluids 2021-09, Vol.175, p.105274, Article 105274
• Main Authors: Fernández-Ponce, M.T., Gómez, E., Cejudo-Bastante, C., Casas, L., Montes, A., Mantell, C., de la Ossa-Fernández, E.J. Martínez, Pereyra, C.
• Format: Article
• Language: English
• Subjects: Alginate; Diabetes mellitus; Mango leaf extract; Supercritical solvent impregnation; Transdermal delivery system
• ISSN: 0896-8446; 1872-8162
• DOI: 10.1016/j.supflu.2021.105274

•Transdermal delivery system with potential to treat Diabetes was developed by SSI.•Mango leaf extract was successfully impregnated into alginate at 200 bar and 35 °C.•SSI of mango extract provides alginate dressings loaded with high phenolic content.•Gallic acid, iriflophenone derivatives and mangiferin were the impregnated compounds.•Impregnated dressings showed antioxidant, antidiabetic and antibacterial activity. [Display omitted] Supercritical solvent impregnation (SSI) has been used to develop a transdermal delivery system consisting of an alginate dressing loaded with mango bioactive compounds. A mango leaf extract (MLE) was produced using CO2 + 50% ethanol at high pressure (200 bar) and temperature (80 °C). The extract presented antidiabetic activity as α-glucosidase inhibition (IC50 = 57.28 μg/mL), antioxidant (EC50 = 10.88 μg/mL), and antibacterial activity against S. aureus (IC50 = 68.77 μg/mL). The influence of impregnation conditions was studied by varying temperature (35–55 °C) and pressure (200–400 bar). Functionalized dressings obtained at 200 bar and 55 °C presented a high antioxidant capacity (~90% DPPH inhibition), as well as antidiabetic (37.5%/10 mg dressing) and antibacterial capacity (64.75%/100 mg dressing). HPLC analysis demonstrated the presence of bioactive compounds such as gallic acid, iriflophenone 3-C-β-D-glucoside, and mangiferin. SEM images showed the presence of MLE particles deposited on the alginate fibers.