Supercritical CO2 with co-solvent extraction of blackberry (Rubus spp. Xavante cultivar) seeds

• Published in: The Journal of supercritical fluids 2022-10, Vol.189, p.105702, Article 105702
• Main Authors: de Souza Correa, Madeline, Boschen, Nayara Lais, Rodrigues, Paulo Rogério P., Corazza, Marcos L., de Paula Scheer, Agnes, Ribani, Rosemary Hoffmann
• Format: Article
• Language: English
• Subjects: Antioxidant; Ethanol; Protocatechuic acid; Rancimat; Rutin
• ISSN: 0896-8446; 1872-8162
• DOI: 10.1016/j.supflu.2022.105702

Blackberry seed extracts (BSEs) were obtained by extraction using supercritical CO2 and ethanol as co-solvent. Conditions of 70 °C and 25 MPa provided the best results: 24.05 wt% of yield, total phenolic content of 3856 mg GAE 100 g−1, and 12,948, 12,996 and 14,243 µmol TE 100 g−1 for the assays ABTS, DPPH and FRAP, respectively. The nutritional lipid indices were PUFA/SFA ratio of 9.60, hypocholesterolemic/hypercholesterolemic ratio of 16.23, atherogenicity 0.06 and thrombogenicity 0.17. The main phenolic compounds were rutin and protocatechuic acid. The thermo-oxidative decomposition of the extract started at 196.25 °C and storage at − 29.52 °C. Rancimat analysis showed that the extract has a protective effect against the oxidation of soybean oil. These results indicate good potential for the application of this extract as an antioxidant. [Display omitted] •Blackberry seed extract (BSE) was obtained using CO2 +ethanol for the first time.•The main phenolic compound present in the BSE was rutin.•The presence of rutin and protocatechuic acid led to high antioxidant activity.•BSE has excellent nutritional lipid indices.