Graphene quantum dots with covalently bonded gold nanoparticles winning the battle against methicillin-resistant Staphylococcus aureus under blue light

• Published in: Synthetic metals 2024-12, Vol.309, p.117753, Article 117753
• Main Authors: Dorontic, Sladjana, Jovanovic, Svetlana, Stefanovic, Andjela, Kepic, Dejan, Scopelliti, Michelangelo, Ciasca, Gabriele, Di Santo, Riccardo, Bogdanovic, Danica Bajuk, Markovic, Olivera, Markovic, Biljana Todorovic, Markovic, Zoran
• Format: Article
• Language: English
• Subjects: Antibacterial agent; Carbodiimide coupling; Graphene quantum dots; Photodynamic therapy; Singlet oxygen production
• ISSN: 0379-6779
• DOI: 10.1016/j.synthmet.2024.117753

Over the last decades, bacterial resistance has become one of the emerging health threats. Particularly dangerous are bacterial strains resistant to various antibacterial drugs. Herein, we modified graphene quantum dots (GQDs) to produce efficient photo-induced antibacterial agents. GQDs were modified with (a) ethylene-diamine (EDA), (b) with EDA and gold nanoparticles (AuNPs), and (c) 3-amino-1,2,4-triazole (TA) using carbodiimide coupling. Photo-induced antibacterial activity of modified GQDs was tested against 8 bacterial strains. Treatment with modified GQDs and blue light (wavelength of 470 nm) resulted in remarkable antibacterial activity with minimal inhibitory concentrations (MIC) of 7.81 µg mL−1 for K. pneumoniae and S. aureus and 3.9 µg mL−1 against MRSA and E. faecalis. Planar organization of GQDs functionalized with AuNPs allowed direct access of molecular oxygen to AuNPs leading to more efficient 1O2 production as well as the 1O2 production from excited GQDs. Thus, GQDs functionalized with AuNPs showed outstanding efficiency in the battle against several bacterial strains, particularly those that lead to nosocomial infections. •Graphene Quantum Dots (GQDs) modified with ethylene-diamine, gold nanoparticles, and triazole.•Carbodiimide coupling was used to bond GQDs with amines and AuNPs.•Modified GQDs exposed to blue light (470 nm) and produced singlet oxygen.•MIC was 7.81 µg mL−1 for K. pneumoniae and S. aureus, and 3.9 µg mL−1 against MRSA and E. faecalis.