Three-dimensional Tri-SNSs-layered electrodeposited reduced graphene oxide for ECL biosensing of DNA

• Published in: Talanta (Oxford) 2018-10, Vol.188, p.58-65
• Main Authors: Wu, Yaoyu, Yang, Jianying, Zheng, Zengyao, Li, Zuoqiu, Lu, Fusheng, Chen, Yaowen, Gao, Wenhua
• Format: Article
• Language: English
• Subjects: 2,2'-Dipyridyl - analogs & derivatives; 2,2'-Dipyridyl - chemistry; 3D-ERGO; Biosensing Techniques - methods; Biosensor; Chitosan - chemistry; Coordination Complexes - chemistry; DNA, Single-Stranded - analysis; DNA, Single-Stranded - chemistry; ECL; Electrochemical Techniques - methods; Electrodes; Graphite - chemistry; Limit of Detection; Luminescence; Luminescent Measurements - methods; Nanostructures - chemistry; Nucleic Acid Hybridization; Oxides - chemistry; Reproducibility of Results; Ru2-DNA; Silver - chemistry; T-DNA; Tri-SNSs
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2018.05.045

In this study,we proposed a triangular silver nanosheets (Tri-SNSs)-layered, Chitosan (CS)-supported three-dimensional of reduced graphene oxide (3D-ERGO) electrochemiluminescence (ECL) biosensing platform using self-designed dual-Ru(bpy)32+ scDNA (Ru2-DNA) as capture probe for ECL biosensing of single-chain DNA (scDNA). Based on the different affinity with scDNA and double chain DNA (dcDNA), the biosensor is designed to recognize the target DNA (t-DNA), which leads to the desorption of a hybrid molecule from the surface of the biosensor, further removing the Ru2-DNA and inhibiting the ECL. Analytical results clearly showed that the electrochemical and ECL behaviors of proposed biosensing platform on the glassy carbon electrode (GCE) exhibited outstanding performance, which was due to large specific surface area, high carrier mobility and strong π–π non-covalent attraction toward single-chain DNA (scDNA) of the stable 3D platform, and ECL amplification of Tri-SNSs. Besides, based on such a system, this strategy can effectively identify full match and mismatched target DNA (M-DNA) with a wide concentration range beyond 7 orders of magnitude and detection limit down to 16.2 aM. Therefore, the 3D biosensing strategy shows potential for the application of bioassays. [Display omitted] •A platform for Ru2-DNA adsorption based on 3D-ERGO is designed.•First to adopt Tri-SNSs as ECL signal amplifier for DNA detection.•Ru2-DNA is synthesized, thereby increasing the ECL signal.•Provide an effective way to distinguish DNA sequence.•This work suggests that the proposed biosensor can be served as a promising choice for bioanalysis.