Simultaneous determination of strobilurin fungicides residues in bean samples by HPLC-UV-AD using boron-doped diamond electrode

• Published in: Talanta (Oxford) 2020-08, Vol.216, p.120957, Article 120957
• Main Authors: Nogueira, Fernanda da Silva, Araujo, Fausto Moreira, De Faria, Lucas Vinícius, Lisboa, Thalles Pedrosa, Azevedo, Gustavo Chevitarese, Dornellas, Rafael Machado, Matos, Maria Auxiliadora Costa, Matos, Renato Camargo
• Format: Article
• Language: English
• Subjects: Amperometry; Bean; Boron - chemistry; Boron-doped diamond; Chromatography, High Pressure Liquid; Electrodes; Fungicides; Fungicides, Industrial - analysis; High performance liquid chromatography; Spectrophotometry, Ultraviolet; Strobilurins; Strobilurins - analysis; Vigna - chemistry
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2020.120957

The aim of this paper was the development of a method for the determination of six strobilurins (fungicides) using boron-doped diamond (BDD) electrode with amperometric detection (AD) homemade coupled to high performance liquid chromatography (HPLC/UV–Vis). HPLC separation of fungicides was performed in a C18 reverse phase column using both UV and AD detectors at 200 mn and 1.9 V, respectively. The linear range for each strobilurin was from 5 to 15 mg L−1 and the correlation coefficients for all the compounds were above 0.997. Both detectors presented adequate detectability (LOD ranging from 1.33 to 1.57 μg kg−1) respecting the limits pre-established by regulatory agencies. The method was validated presenting good values of recovery and accuracy. In the spiked samples the recoveries ranged from 61.6% (trifloxystrobin) to 98.8% (azoxystrobin) for UV and 62.3% (trifloxystrobin) to 95.2% (azoxystrobin) for AD. In blanks spikes the recovery varied from 77.8% (picoxystrobin) to 88.4% (kresoxim-methyl) for UV and 76.7% (picoxystrobin) to 87.1% (dimoxystrobin) for AD. The method showed good precision (RSD