Enzymatic synthesis of N-methyl- l-phenylalanine by a novel enzyme, N-methyl- l-amino acid dehydrogenase, from Pseudomonas putida

[Display omitted] An enzymatic system for the synthesis of N-methyl- l-phenylalanine from phenylpyruvic acid and methylamine with a novel enzyme, N-methyl- l-amino acid dehydrogenase from Pseudomonas putida ATCC12633, using NADP + and glucose dehydrogenase from Bacillus subtilis as a co-factor-recyc...

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Bibliographic Details
Published in:Tetrahedron: asymmetry 2004-09, Vol.15 (18), p.2841-2843
Main Authors: Muramatsu, Hisashi, Mihara, Hisaaki, Kakutani, Ryo, Yasuda, Mari, Ueda, Makoto, Kurihara, Tatsuo, Esaki, Nobuyoshi
Format: Article
Language:English
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Summary:[Display omitted] An enzymatic system for the synthesis of N-methyl- l-phenylalanine from phenylpyruvic acid and methylamine with a novel enzyme, N-methyl- l-amino acid dehydrogenase from Pseudomonas putida ATCC12633, using NADP + and glucose dehydrogenase from Bacillus subtilis as a co-factor-recycling system is described. Analysis of the product on a laboratory preparative scale process revealed N-methyl- l-phenylalanine in 98% yield and over 99% ee. N-Methyl- l-phenylalanine can be used as chiral building blocks for the synthesis of several products with pharmacological activity.
ISSN:0957-4166
1362-511X
DOI:10.1016/j.tetasy.2004.06.030