A novel fluorescent guanine derivative distinguishable of three structures, single strand, duplex, and quadruplex

Graphic We have constructed a pyrene-labeled guanine base, 8PyG. 8PyG is a novel fluorescent probe for monitoring the secondary structure of G-rich DNA. The fluorescence emitted from 8PyG-labeled oligodeoxynucleotide clearly distinguished three structural states, single strand–duplex–quadruplex. Thu...

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Bibliographic Details
Published in:Tetrahedron letters 2004-07, Vol.45 (31), p.6059-6062
Main Authors: Okamoto, Akimitsu, Kanatani, Keiichiro, Ochi, Yuji, Saito, Yoshio, Saito, Isao
Format: Article
Language:English
Subjects:
Fluorescence
Oligodeoxynucleotide
Pyrene
Quadruplex
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Summary:Graphic We have constructed a pyrene-labeled guanine base, 8PyG. 8PyG is a novel fluorescent probe for monitoring the secondary structure of G-rich DNA. The fluorescence emitted from 8PyG-labeled oligodeoxynucleotide clearly distinguished three structural states, single strand–duplex–quadruplex. Thus, the technique, which monitors the fluorescence of 8PyG-labeled oligodeoxynucleotide, is a powerful tool for the investigation of DNA structural changes.
ISSN:0040-4039
1873-3581
DOI:10.1016/j.tetlet.2004.05.136