Immunization against Kisspeptin-54 perturb hypothalamic–pituitary–testicular signaling pathway in ram lambs

Kisspeptin, a peptide product of KISS1 gene, recently identified as essential upstream gatekeeper in hypothalamic-pituitary-gonadal (HPG) axis. This study was designed to investigate the effect of immunization against kisspeptin-54 on hypothalamic-pituitary-testicular signaling pathway. A total of t...

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Bibliographic Details
Published in:Theriogenology 2019-02, Vol.125, p.193-202
Main Authors: Wassie, Teketay, Liu, Guiqiong, Jiang, Xunping, Tesema, Birhanu, Han, Yanguo, Zhao, Jiayu, Girmay, Shishay, Ahmad, Hafiz Ishfaq
Format: Article
Language:English
Subjects:
Anti-kisspeptin antibody
Antifertility vaccine
KISS1
Kisspeptin-54
Ram
Signaling pathway
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Summary:Kisspeptin, a peptide product of KISS1 gene, recently identified as essential upstream gatekeeper in hypothalamic-pituitary-gonadal (HPG) axis. This study was designed to investigate the effect of immunization against kisspeptin-54 on hypothalamic-pituitary-testicular signaling pathway. A total of ten intact 56-days-old ram lambs were used and randomized into the treatment and control groups, which were, respectively immunized by kisspeptin-54 based vaccine and the empty plasmid via intramuscular route. We employed indirect enzyme-linked immunosorbent assay and quantitative real-time PCR to characterize the difference in serum kisspeptin, luteinizing hormone, testosterone hormone concentration and mRNA expression of reproductive-related genes in HPG axis across kisspeptin-54 immunized and control ram lambs. Serum kisspeptin, luteinizing hormone and testosterone concentration in the treatment group was lower (p 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2018.10.029