Effects of EPA on bovine oocytes matured in vitro with antioxidants: Impact on the lipid content of oocytes and early embryo development

The eicosapentaenoic acid (EPA) is an n-3 polyunsaturated fatty acid (PUFA) present in the lipid composition of bovine oocytes. Little is known about the importance of EPA in bovine oocyte maturation and embryo development in vitro. Although previous work suggest that n-3 PUFAs may inhibit oocyte ma...

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Bibliographic Details
Published in:Theriogenology 2020-04, Vol.146, p.152-161
Main Authors: Nikoloff, Noelia, Campagna, Anabella, Luchetti, Carolina, Carranza-Martín, Ana C., Pascua, Ana M., Anchordoquy, Juan Mateo, Anchordoquy, Juan Patricio, Lombardo, Daniel M., Seoane, Analia, Furnus, Cecilia C.
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Cattle
Cysteamine
Cysteamine - administration & dosage
Cysteamine - pharmacology
Cystine Depleting Agents - administration & dosage
Cystine Depleting Agents - pharmacology
Eicosapentaenoic Acid - administration & dosage
Eicosapentaenoic Acid - pharmacology
Embryo Culture Techniques - veterinary
Embryonic Development - drug effects
EPA
In Vitro Oocyte Maturation Techniques - veterinary
Lipid droplets
Lipids - chemistry
Oocyte maturation
Oocytes - chemistry
Oocytes - drug effects
Oxidative stress
Vitamin E
Vitamin E - administration & dosage
Vitamin E - pharmacology
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Summary:The eicosapentaenoic acid (EPA) is an n-3 polyunsaturated fatty acid (PUFA) present in the lipid composition of bovine oocytes. Little is known about the importance of EPA in bovine oocyte maturation and embryo development in vitro. Although previous work suggest that n-3 PUFAs may inhibit oocyte maturation, the available data are inconsistent. In this study, we evaluated the effect of EPA (1, 10, 100 nM) during in vitro maturation (IVM) of bovine oocytes, alone and in combination with vitamin E (VE) or cysteamine (CYS). EPA treatment in IVM decreased oocyte lipid content and affected lipid droplets pattern (P 
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2019.11.028